文献类型：期刊文献

中文题名：重组大肠杆菌发酵生产核酸疫苗的初步研究

英文题名：A Preliminary Study of Fermentation Conditions for Producing DNA Vaccine by Recombinant Escherichia coli

作者：柯伟[1];夏杰[1];陆兵[1];徐殿胜[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2006

卷号：32

期号：1

起止页码：38

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：核酸疫苗;质粒;培养基;流加培养;均匀设计

外文关键词：DNA vaccine ; plasmid ; medium ; fed-batch ; uniform design

摘要：初步摸索大肠杆菌DH 5α生产核酸疫苗的发酵条件。通过均匀试验设计确定,当胰蛋白胨与酵母粉的质量比为1∶1.2,并且磷酸盐的加量较大时菌体的质粒产量较高。在菌体生长刚进入对数生长期时采用指数式流加葡萄糖与采用恒速流加葡萄糖,菌体得率相同,而产物收率前者高于后者。在菌体生长进入平稳期时将温度由37°C升高到42°C,在菌体密度没有改变的情况下,质粒产量得到提高,达到75 m g/L。
The fermentation conditions of Escherichia coli DH5α for the production of tuberculosis DNA vaccine were studied. The method of uniform design being applied to the design of culture medium, it was found that the productivity of the plasmid DNA per cell was optimal when the ratio of trypton and yeast extract was 1 ： 1.2 and the quantity of phosphate added was adequate. Campared with the fed-batch operation with constant glucose feed rate, the fed-batch operation with exponential glucose feed rate has an increased yield of plasmid DNA per cell while the yield of cell per gram of glucose remains the same. The stepwise increase of the culture temperature from 37℃ to 42℃ in the stationary phase enhanced the productivity of plasmid DNA to 75 mg/L while the biomass concentration was the same in the process with constant temperature of 37℃.