文献类型：期刊文献

英文题名：Enhancement of the surface free energy of PDMS for reversible and leakage-free bonding of PDMS-PS microfluidic cell-culture systems

作者：Song, Ki-Young[1,2];Zhang, Hongbo[4];Zhang, Wen-Jun[3];Teixeira, Ana[1]

机构：[1]Karolinska Inst, Dept Med Biochem & Biophys, Stockholm, Sweden;[2]Beijing Inst Technol, Sch Mechatron Engn, Beijing, Peoples R China;[3]Univ Saskatchewan, Dept Mech Engn, Saskatoon, SK, Canada;[4]East China Univ Sci & Technol, Sch Mech & Power Engn, Shanghai, Peoples R China

年份：2018

卷号：22

期号：11

外文期刊名：MICROFLUIDICS AND NANOFLUIDICS

收录：;EI(收录号：20184606078015);WOS:【SCI-EXPANDED(收录号:WOS:000449826300001)】；

基金：This work was supported by grants from the Human Frontier Science Program Young Investigator Award (AIT, RGY0082-2014), the Swedish Research Council (AIT; 2015-03520), NSERC Discovery Grant of Canada.

语种：英文

外文关键词：PDMS; Polystyrene; Bonding; Surface free energy; Microfluidic cell-culture systems

摘要：Polydimethylsiloxane (PDMS) has become one of the most widely used materials in the fabrication of microfluidic systems bonded onto glass substrates, especially for cell biology applications. However, PDMS is often unsuitable for building microfluidic systems onto polystyrene (PS) which is the preferred substrate in most cell-culture protocols. In particular, PS is required for culturing many stem cell and primary cell types. Here, we propose a novel approach to building PDMS-PS microfluidic cell-culture systems, specifically realizing a strong and reversible bonding of PDMS on PS without using chemical agents which can have negative effects on cell viability. Our strategy to strengthen the bonding of PDMS to PS surfaces is to increase the surface free energy (SFE) by adjusting the mixing ratio of PDMS base to curing agent and by treating the surfaces of PDMS and PS with O-2 plasma and annealing. Our results show that using this method for PDMS-PS bonding, we are able to produce reliable reversible and leakage-free PDMS-PS microfluidic cell-culture systems.