文献类型：期刊文献

英文题名：A Carbapenem-Based Off-On Fluorescent Probe for Specific Detection of Metallo-β-Lactamase Activities

作者：Mao, Wuyu[1];Wang, Yaqun[1];Qian, Xiana[1];Xia, Lingying[1];Xie, Hexin[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China

年份：2019

卷号：20

期号：4

起止页码：511

外文期刊名：CHEMBIOCHEM

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000458825800009)】；

基金：We are grateful for financial support from the National Natural Science Foundation of China (21472048), the "Thousand Plan" Youth Program, the Fundamental Research Funds for the Central Universities, and East China University of Science & Technology.

语种：英文

外文关键词：antibiotics; fluorescent probes; lactams; metalloenzymes; sensors

摘要：Metallo-beta-lactamase is one of the major clinical threats because this beta-lactam-hydrolyzing enzyme confers significant resistance to most beta-lactam antibiotics, including carbapenems, among bacterial pathogens. Reported herein is a novel fluorogenic sensor for the specific detection of metallo-beta-lactamase activities. This carbapenem-based reagent exhibits excellent selectivity to metallo-beta-lactamase over other serine-beta-lactamases, including serine carbapenemases. The usefulness of this probe was further demonstrated in the rapid identification of metallo-beta-lactamase-expressing pathogenic bacteria.