文献类型：期刊文献

英文题名：Effect of mobile phase composition and pH on HPLC separation of rhizome of Polygonum bistorta

作者：Intisar, Azeem[1]; Kiazolu, J. Boima[1]; Wang, Yun[1]; Zhang, Lingyi[1]; Zhang, Weibing[1]

机构：[1] Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, China

年份：2012

卷号：35

期号：7

起止页码：977

外文期刊名：Journal of Liquid Chromatography and Related Technologies

收录：EI(收录号：20121614957419)

语种：英文

外文关键词：Phase composition - Organic acids - Extraction

摘要：A simple, sensitive, and reliable HPLC method was developed for the overall separation of different chemical constituents present in the rhizome of Polygonum bistorta. The extraction conditions including soaking, sonication for 30 minutes, and heating for one hour were selected to achieve complete extraction. Different chromatographic conditions such as mobile phase composition and pH were studied and compared in the Reversed Phase separation mode to acquire fingerprinting profiles. 30 distinct peaks were obtained and 3 phenolic acids (gallic acid, protocatechuic acid, and chlorogenic acid) with anticancer bioactivity were identified by comparing their retention times with reference marker compounds in the optimized method. The excellent repeatability was tested by calculating relative standard deviations (RSDs) for retention times and peak areas and resultant values were less than 0.5% and 1.2%, respectively, for these respective compounds. This method can be used for quality assessment and further identification, quantification, and purification of the important bioactive constituents present in the rhizome of Polygonum bistorta. Copyright ? Taylor & Francis Group, LLC.