详细信息
文献类型:期刊文献
中文题名:基于微通道的ZnO纳米棒生物荧光检测研究
英文题名:Study of bioluminescence detection of ZnO nanorods based on microchannels
作者:刘向飞[1];蒋栋[2];赵振杰[1];李欣[1]
机构:[1]华东师范大学纳光电集成与先进装备教育部工程研究中心,上海200062;[2]华东理工大学分析测试中心,上海200237
年份:2015
卷号:34
期号:5
起止页码:22
中文期刊名:传感器与微系统
外文期刊名:Transducer and Microsystem Technologies
收录:CSTPCD;;CSCD:【CSCD_E2015_2016】;
基金:国家自然科学基金资助项目(51302085);华东师范大学创新基金资助项目(78210142;78210183)
语种:中文
中文关键词:微通道;Zn;O纳米棒;生物荧光检测
外文关键词:microchannel ; ZnO nanorods ; bioluminescence detection
摘要:利用种子法和水热合成技术,分别在常规条件下和阵列式微通道中制备氧化锌(Zn O)纳米棒。采用扫描电子显微镜(SEM)、X射线衍射(XRD)等分析方法表征Zn O纳米棒的表面形貌特点和晶体结构。结果表明:微通道中制备的Zn O纳米棒的比表面积、结晶度和c轴取向性均有较大程度的提高。同时,建立了基于阵列式微通道的Zn O纳米棒生物荧光检测方法,利用Zn O纳米棒可显著增强荧光信号,对异硫氰酸荧光素标记的羊抗牛Ig G抗体的检测限为1×10-4μg/m L。
The ZnO nanorods are hydrothermally grown starting from a ZnO seed layer with conventional method and in microchannel arrays. The structures of prepared ZnO nanorods are characterized using a scanning electron microscopy( SEM) and their crystalline properties are analyzed by X-ray diffraction( XRD). The results show that the surface area,crystalline and c-axis orientation of ZnO nanorods have been improved significantly. Meanwhile,bioluminescence detection of ZnO nanorods has been established based on microchannel arrays,and fluorescence signals have been enhanced on ZnO nanorods platforms and detection limit of FITC-anti IgG is 1 × 10^-4μg / m L.
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