文献类型：期刊文献

英文题名：A facile transport assay for H+ coupled membrane transport using fluorescence probes

作者：Lan, Wanjun[1]; Ren, Hongliu[1]; Pang, Yu[1]; Huang, Chuseng[1]; Xu, Yufang[1]; Brooker, Robert J.[2]; Zhang, Jingyan[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China; [2] Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, United States

年份：2012

卷号：4

期号：1

起止页码：44

外文期刊名：Analytical Methods

收录：EI(收录号：20120214679342)

语种：英文

外文关键词：Ion exchange - Fluorescence - Metal ions - Metals

摘要：A facile activity assay for an H+-coupled transporter using florescent probes was developed with an H+-coupled manganese transporter (MntH) as a model. Making use of coupled-proton transport, the transport activity (H+/Mn2+ cotransport) can be directly determined via fluorescence intensity changes of the probe, 5-(and-6)- carboxyfluorescein (5(6)-FAM). The approach of using highly sensitive fluorescence probes provides a more simple and convenient assay method for the determination of proton-coupled metal-ion uptake by transporters. ? 2012 The Royal Society of Chemistry.