文献类型：期刊文献

英文题名：Enhancement of dalesconols A and B production via upregulation of laccase activity by medium optimization and inducer supplementation in submerged fermentation of Daldinia eschscholzii

作者：Pan, Zheng-Hua[1,2];Jiao, Rui-Hua[3];Lu, Yan-Hua[1,2];Tan, Ren-Xiang[3]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg, Shanghai 200237, Peoples R China;[3]Nanjing Univ, State Key Lab Pharmaceut Biotechnol, Inst Funct Biomol, Nanjing 210008, Jiangsu, Peoples R China

年份：2015

卷号：192

起止页码：346

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20152400929152);WOS:【SCI-EXPANDED(收录号:WOS:000357664200046)】；

基金：This work is financially supported by the National High Technology Research and Development Program of China (2013AA092901), the Fundamental Research Funds for the Central Universities, as well as the National Special Fund for State Key Laboratory of Bioreactor Engineering (2060204).

语种：英文

外文关键词：Dalesconols A; Dalesconols B; Laccase activity; Submerged fermentation; Daldinia eschscholzii

摘要：Dalesconols (dalesconols A and B) are novel polyketides with strong immunosuppressive activity produced by Daldinia eschscholzii. In this work, the effects of different media (M1, M2, and M3) on fungus growth and dalesconols biosynthesis were firstly tested and compared. Intermediates and enzyme analysis indicated that laccase had the major contribution to dalesconols biosynthesis. The key role of laccase on dalesconols biosynthesis was further experimentally confirmed, which suggested that the modified M2 was more favored for laccase and dalesconols production. Thereafter, the medium composition was optimized by RSM with a fermentation titer of 36.66 mg/L obtained. Furthermore, Ca2+ induction was employed to up-regulate of laccase activity and further enhanced dalesconols production (76.90 mg/L), which was 308% higher than that in M2. In addition, dalesconols production reached 63.42 mg/L in scale-up experiments. This work indicated great potential of laccase as a key enzyme on regulation of dalesconols production. (C) 2015 Elsevier Ltd. All rights reserved.