文献类型：期刊文献

中文题名：Aerolysin单分子界面的构建及高选择性单分子检测

英文题名：Construction and High Selectivity of Aerolysin Single Molecular Interface for Single Molecule Analysis

作者：武雪原[1];应佚伦[1,2];龙亿涛[1,2]

机构：[1]华东理工大学化学与分子工程学院,上海200237;[2]南京大学化学化工学院,生命分析化学重点实验室,南京210023

年份：2019

卷号：40

期号：9

起止页码：1825

中文期刊名：高等学校化学学报

外文期刊名：Chemical Journal of Chinese Universities

收录：CSTPCD;;EI(收录号：20194607699201);Scopus;WOS:【SCI-EXPANDED(收录号:WOS:000492677600006)】；北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：Supported by the National Natural Science Foundation of China(Nos. 21834001, 61871183).

语种：中文

中文关键词：气单胞菌溶素;单分子界面;纳米孔道;单分子分析

外文关键词：Aerolysin;Single molecular interface;Nanopores;Single molecule analysis

摘要：提出了一种基于Aerolysin膜蛋白质分子构建单分子界面的方法,运用蛋白质工程技术对单分子界面进行定点修饰,所建立方法灵活、可控且重复性好.采用Poly(dA)4为探针分子对修饰后的单分子界面进行了表征,结果表明,在孔口处的Arginine修饰影响了寡聚核苷酸的选择性.为进一步理解Aerolysin单分子界面及合理设计功能性单分子界面提供了参考.
As an new conceptual single molecular interface, the biomolecular nanopore can accommodate only a single molecule and contains abundant amino acid residues in the lumen, providing an appropriate confinement for single molecule detection. Herein, a generalized method is proposed for the construct of single molecular interface based on membrane protein. The method is flexible, controllable and repeatable. The characterization of D209R mutation with Poly(dA) 4 illustrate that the selectivity of aerolysin single molecular interface is related the electrostatic properties at the entrance of nanopore, which deepens our understanding of aerolysin single molecular interface, providing a paradigm for rational design of functional single molecular interface.