文献类型：期刊文献

英文题名：Fluorinated plant activators induced dual-pathway signal transduction and long-lasting ROS burst in chloroplast

作者：Shi, Qinjie[1];Fu, Jianmian[1];Zhou, Yiqing[3];Ji, Yuanyuan[1];Zhao, Zhenjiang[1];Yang, Yangyang[1];Xiao, Youli[3];Qian, Xuhong[1,2];Xu, Yufang[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[3]Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Inst Plant Physiol & Ecol, CAS Key Lab Synthet Biol, Shanghai 200032, Peoples R China

年份：2024

卷号：204

外文期刊名：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001291723500001)】；

基金：This work is supported by National Key Research and Development Program of China (2022YFD1700403 and 2023YFD1700303) .

语种：英文

外文关键词：Plant activator; Systemic acquired resistance; Induced systemic resistance; Plant immune system

摘要：Synthetic plant activators represent a promising novel class of green pesticides that can triggering endogenous plant immunity against pathogen invasion. In our previous study, we developed a series of fluorinated compounds capable of eliciting disease resistance in plants; however, the underlying regulatory mechanisms remained unclear. In this study, we systematically investigated the mechanism of plant immune activation using four synthetic plant activators in Arabidopsis thaliana (A. thaliana), including two fluorine-substituted and two non-fluorine-substituted molecules. Our findings revealed that the fluorinated compounds exhibited superior disease resistance activity compared to the non-fluorinated molecules. Gene expression analysis in systemic acquired resistance (SAR)- and induced systemic resistance (ISR)-related pathways demonstrated that fluorine substitution effectively regulated both SAR- and ISR-pathway activation, highlighting the distinct roles of fluorine in modulating the plant immune system. Notably, the prolonged ROS burst was observed in chloroplasts following treatment with all four plant activators, contrasting with the transient ROS burst induced by natural elicitors. These results provide insights into the unique mechanisms underlying synthetic plant activator-induced plant immunity. Furthermore, comprehensive proteomic analysis revealed a robust immune response mediated by fluorine-substituted plant activators. These findings offer novel insights into the role of fluorine substitution in SAR- and ISR-associated immune signaling pathways and their distinct impact on ROS production within chloroplasts.