详细信息
乳酸菌菌种的简便分离和培养
文献类型:期刊文献
中文题名:乳酸菌菌种的简便分离和培养
作者:周家春[1]
机构:[1]华东理工大学生物工程学院,上海200237
年份:1998
卷号:19
期号:1
起止页码:39
中文期刊名:食品科学
外文期刊名:Food Science
收录:CSTPCD;;Scopus;北大核心:【北大核心1996】;CSCD:【CSCD2011_2012】;
语种:中文
中文关键词:乳酸菌;分离;菌种分离;酸乳制品
外文关键词:Lactic acid bacteria;Separation
摘要:嗜热链球菌和保加利亚杆菌是酸乳制品的常用菌种,因其有共生关系,较难分离。用特殊的培养基如M17和MRS酸化培养基可以将此共生苗分离。本文介绍的培养基同样能起到分离的作用,且成分简单,取材方便。
The best composition of the culture medium of streptococcus thermophilus is: skimmed milk powder13%, glucose 2%, hydrolyzed milk powder 4%, yeast extract 0. 25%, phosphate buffer 0. 025%mol/L,PH6. 8. It would get Peak strain concentration when streptococcus thermophilus was cultured at 37℃ for 6-8hours. And for lactobacillus bulgaricus, they are: skimmed milk powder 13%, glucose 2%, yeast extract0. 25%, phosphate buffer 0. 05mol/L, PH 6. 8, culture at 42℃ for 6 hours.
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