文献类型：期刊文献

英文题名：Bacterial infection induces pyroptotic signaling-mediated neutrophil extracellular traps (NETs) formation in turbot (Scophthalmus maximus)

作者：Zhao, Jingjing[1];Chen, Weijie[1];Zhang, Yuanxing[2,3];Liu, Qin[1,3];Yang, Dahai[1,3];Wang, Zhuang[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China;[3]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China

年份：2022

卷号：127

起止页码：982

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001084587300003)】；

基金：This work was supported by the National Natural Science Foundation of China (32102836), the Natural Science Foundation of Shanghai (20ZR1415500) and the Fundamental Research Funds for the Central Universities (222201221733).

语种：英文

外文关键词：Bacterial infection; Neutrophil; Neutrophils extracellular traps; Pyroptosis; Turbot

摘要：Neutrophils can capture and kill pathogens by releasing neutrophils extracellular traps (NETs), which play critical roles in anti-microbial infection in mammals; however, the mechanisms involved in NETs formation and its role in anti-bacterial infection in teleost fish remains largely unknown. In this study, to explore the function of NETs in turbot, we established an in vitro bacterial infection model in head kidney derived neutrophils, and found that the haemolysin over-expressed Edwardsiella piscicida (ethA(+)) could induce a robust phenotype of NETs, compared with that in wild type or ethA mutant (ethA(+) -Delta ethA) strains. Besides, the NETosis was mediated by ethA(+) -induced pyroptosis, and arms the ability of bacterial killing in neutrophils of turbot. Moreover, we found that neutrophils elastase (NE) might involves in this pyroptotic signaling, rather than inflammatory Smcaspase. Taken together, this study reveals the important role of pyroptosis in NETs formation in turbot neutrophils, suggesting that NETs formation is a critical immune response during bacterial infection in teleost fish.