文献类型：期刊文献

中文题名：海洋放线菌Y12-26代谢产物中bafilomycins分离纯化及结构鉴定

英文题名：Isolation,purification and structure identification of secondary metabolites produced by marine actinomycete Y12-26

作者：魏刚[1];苏超[1];张道敬[2];宗志友[3];斯聪聪[1];陶黎明[1]

机构：[1]华东理工大学上海市化学生物学(芳香杂环)重点实验室,上海200237;[2]华东理工大学生物反应器工程国家重点实验室,上海200237;[3]上海师范大学生命与环境科学学院,上海200234

年份：2011

卷号：36

期号：8

起止页码：571

中文期刊名：中国抗生素杂志

外文期刊名：Chinese Journal of Antibiotics

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家高科技研究发展(863)计划(编号:2008AA09Z407)

语种：中文

中文关键词：海洋放线菌;分离纯化;结构鉴定;bafilomycins

外文关键词：Marine actinomycete; Isolation and purification; Structure identification; Bafilomycins

摘要：目的了解海洋放线菌Y12-26的次级代谢产物中对稻瘟病菌(Pyricularia oryzae)等植物病原真菌具有较好的拮抗活性的具体化合物。方法以P.oryzae指示菌进行活性跟踪,对其代谢物进行分离提取,通过有机溶剂萃取、硅胶柱层析、TLC和HPLC制备等方法分离得到活性化合物,并通过UV、MS、NMR等方法进行结构鉴定。结果从海洋放线菌Y12-26发酵物中分离得到了3个大环内酯类活性化合物Y12-26-A2a、A3a和A3b,确定Y12-26-A2a分子式为C35H56O8,与bafilomycin D结构相同;Y12-26-A3a分子式为C35H58O9,与bafilomycin A1结构相同;Y12-26-A3b为新结构化合物,分子式为C35H58O8,命名为bafilomycin K。结论化合物Y12-26-A2a、A3a和A3b对P.oryzae的MIC分别为125、16和31μg/mL。
Objective To know the active components in the secondary metabolites produced by marine actinomycete Y12-26 exhibited antagonistic activity against Pyricularia oryzae. Methods By bioassay-guided isolation and purification, the active antifungal compounds were isolated and purified from the culture extract of strain Y12-26 with solvent extract, silica gel chromatography, thin layer chromatography, and high performance liquid chromatography and other ways, and their structure were elucidation with the help of spectra data analysis ofUV, ESI- MS, NMR and others. Results Three antifungal macrolide compounds Y 12-26-A2a, A3a and A3b were isolated from the culture extract of strain Y12-26. the molecular formula of the compound Y12-26-A2a was confirmed as C35H5608, as the same as the known compound bafilomycin D. The molecular formula of the compound Y12-26-A3a was confirmed as C35H58O9, the structure is the same as bafilomycin A1. The molecular formula of the compound Y12-26- A3b was confirmed as C35H58O8, which was identificated as a new compound named bafilomycin K. Conclusion The MIC of the three compoundsY12-26-A2a, A3a and A3b against P oryzae were 125, 16 and 31μg/mL.