文献类型：期刊文献

英文题名：Enhanced bacteriostatic activity, osteogenesis and osseointegration of silicon nitride/polyetherketoneketone composites with femtosecond laser induced micro/nano structural surface

作者：Wu, Han[1];Liu, Tao[2];Xu, Zhiyan[1];Qian, Jun[1];Shen, Xuening[1];Li, Yuan[1];Pan, Yongkang[1];Wang, Deqiang[1];Zheng, Kai[3];Boccaccini, Aldo R.[3];Wei, Jie[1]

机构：[1]East China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[2]Jiangwan Hosp, Dept Orthopaed, Shanghai 200434, Hongkou Distric, Peoples R China;[3]Univ Erlangen Nurnberg, Dept Mat Sci & Engn, Inst Biomat, Erlangen 91058, Germany

年份：2020

卷号：18

外文期刊名：APPLIED MATERIALS TODAY

收录：;EI(收录号：20195007830204);WOS:【SCI-EXPANDED(收录号:WOS:000530651100020)】；

基金：This work was supported by the National Natural Science Foundation of China (grant Nr.: 81572194 and 81771990), Technology Development of Shanghai (grant Nr.: 17441900600 and17441902000), Clinical Medicine Excellent Youth Talents Training Program og Hongkou District of Shaghai (HKYQ2018-13), Medical Scientific Research Foundation of Hongkou District Health and Family Planning Commission (HW1802-19). The authors also thank German Academic Exchange Service (DAAD) and China Scholarship Council (CSC) for the funding of the collaborative project "DAADProgramme des Projektbezogenen Personenaustauschs (DAADPPP) Project Nr.: 57389773".

语种：英文

外文关键词：Femtosecond laser; Silicon nitride; Polyetherketoneketone; Micro/nano structure; Osseointegration

摘要：Poor cell adhesion and osteogenic activity impede the bone regeneration and osseointegration effects of polyetherketoneketone (PEKK) implants. Surface modification and incorporation of bioactive fillers are effective strategies to promote the cellular responses of PEKK implants and their integration with bone tissues. In this study, silicon nitride (SN) microparticles were blended with PEKK to develop bioactive composite implants (SPC). Femtosecond laser was then used to modify SPC surfaces inducing favorable micro/nano structural surface (FSPC). After the laser ablation, irregularly shaped SN microparticles were converted to "petal-like" clusters composed of "pin-like" SN nanoparticles, while the entire surface was covered by SN particles due to the removal of PEKK. The roughness, hydrophilicity, content of surface chemical groups (e.g., -NH2), and protein adsorption capability of FSPC were significantly enhanced compared to SPC and PEKK. Also, the release of biologically active Si ions was increased after the femtosecond laser treatment, inducing a positive microenvironment favorable for cellular activities. Moreover, FSPC exhibited a greater bacteriostatic activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) than SPC. The presence of micro/nano structure also remarkably promoted adhesion, proliferation, and osteogenic differentiation of rat bone mesenchymal stem cells (rBMSCs). In vivo evaluation of the composites in a rabbit femur defect model verified that FSPC could enhance osteogenesis and osseointegration to a greater extent than SPC, evidenced with greater bone-implant contacts and push-out force. These results indicate that the femtosecond laser induced micro/nano structural surface on SN/PEKK implants can significantly promote osseointegration and bone repair. The femtosecond laser exhibits great potential in surface modifying bioceramic/polymer composites. The fabricated FSPC have shown great potential as bone implants in orthopedic applications considering their excellent biocompatibility, bacteriostatic property, osteogenic activity, and osseointegration. (C) 2019 Elsevier Ltd. All rights reserved.