文献类型：期刊文献

英文题名：Visualizing glioma margins by real-time tracking of γ-glutamyltranspeptidase activity

作者：Liu, Yingchao[1];Tan, Jie[2,3];Zhang, Yi[4];Zhuang, Jianfeng[1];Ge, Mingxu[1];Shi, Ben[2,3];Li, Jiao[2,3];Xu, Ge[2,3];Xu, Shangchen[1];Fan, Chunhai[5];Zhao, Chunchang[2,3]

机构：[1]Shandong Univ, Prov Hosp, Dept Neurosurg, Jinan 250021, Shandong, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Sch Chem & Mol Engn, Inst Fine Chem, Shanghai 200237, Peoples R China;[4]Shandong Univ, Shandong Med Imaging Res Inst, Jinan 250021, Shandong, Peoples R China;[5]Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai 201800, Peoples R China

年份：2018

卷号：173

起止页码：1

外文期刊名：BIOMATERIALS

收录：;EI(收录号：20182205252851);WOS:【SCI-EXPANDED(收录号:WOS:000436054300001)】；

基金：We gratefully acknowledge the financial support from the National Natural Science Foundation of China (Grant Nos. 21672062, 21372083, 21574044, 81641176), the Natural Science Foundation of Shandong Province (ZR2014HM069), the Science and Technology Planning Project of Shandong Province (2014A010105009, 2013CB834702, 2014GSF118046), the 2016 National Key Research and Development Program (2016YFC0106105) and the Taishan scholars program (NO. tsqn20161070).

语种：英文

外文关键词：Glioma margins; Activatable probe; gamma-Glutamyltranspeptidase; Real-time; Clinical

摘要：Distinguishing tumor from adjacent non-cancerous tissue can be problematic during surgical treatment of malignant glioma. Consequently, a novel approach to selective discrimination is required. The goal of this study was to determine whether a fluorescent probe activated by gamma-Glutamyltranspeptidase (GGT), an enzyme that is overexpressed on glioma cell membranes but only minimally expressed in normal brain tissue, could be used to visualize glioma margins. Here, we showed that the GGT-activatable fluorescent probe (NC-B-Cys-gamma-Glu) provided real-time in situ tracking of enzyme activity that accurately distinguished glioma from healthy brain tissue. NC-B-Cys-gamma-Glu, which featured distinct ratiometric fluorescence responsiveness after interaction with GGT, enabled monitoring of GGT activity in living cells and differentiation between glioma and normal cells. Topical spraying of NC-B-Cys-gamma-Glu facilitated real-time in vivo identification of orthotopic glioblastomas in a mouse model. Importantly, the tumor, infiltrating area and surrounding normal tissue were distinguished in clinical glioma samples by real-time tracking of GGT activity. When coupled with auto fluorescence bronchoscopy, NC-B-Cys-gamma-Glu offered diagnostic value for cancers overexpressing GGT. Therefore, NC-B-Cys-gamma-Glu might offer a promising tool to guide maximal yet precise tumor resection while sparing non-cancerous tissue. (C) 2018 Elsevier Ltd. All rights reserved.