文献类型：期刊文献

英文题名：DNAzyme-based microarray for highly sensitive determination of metal ions

作者：Zuo, Peng[1];Yin, Bin-Cheng[1];Ye, Bang-Ce[1]

机构：[1]E China Univ Sci & Technol, Dept Food Engn & Sci, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China

年份：2009

卷号：25

期号：4

起止页码：935

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：;EI(收录号：20094512417557);WOS:【SCI-EXPANDED(收录号:WOS:000272439800045)】；

基金：The authors thank Prof. Yi Lu of the University of Illinois at Urbana-Champaign for many helpful suggestions and discussion. This work was financially supported by China NSF (20627005 and 20776039), Shanghai Project (07dz19508 and 09JC1404100), SKLBE Fund (2060204). NCET-07-0287, and Shuguang 06SG32.

语种：英文

外文关键词：DNA cleavage; DNAzyme; Heavy metal ions; Microarray; Multiplex determination

摘要：A novel microarray for the multiplex determination of heavy metal ions in aqueous solution based on DNAzymes has been developed with good sensitivity and selectivity. In the present work, metal ion dependent DNAzymes of copper (Cu-Enz) and lead (Pb-Enz) were first associated with their corresponding DNA substrates (Cu-Sub and Pb-Sub) immobilized on the surface of aldehyde-coated slides. After introducing the corresponding metal ions, the DNA cleavage of the substrates caused by the DNAzymes took place, resulting in a dramatic change in fluorescent signal intensity. The proposed microarray method, which can be used as a multi-component assay with high efficiency, combines the high sensitivity and selectivity of DNAzymes with the high throughput and parallel analysis of microarray technology. The method reveals a sensitivity corresponding to 0.6 ppb and 2 ppb for Cu2+ and Pb2+, respectively, which is sufficient to detect them in drinking water. This approach may find potential applications in environmental monitoring, food safety monitoring, clinical toxicology, waste treatment, the cosmetic industry and industrial process monitoring. (C) 2009 Elsevier B.V. All rights reserved.