文献类型：期刊文献

英文题名：In situ bone regeneration enabled by a biodegradable hybrid double-network hydrogel

作者：Zhang, Yuanhao[1];Chen, Mingjiao[2];Tian, Jia[1];Gu, Ping[2];Cao, Hongliang[1];Fan, Xianqun[2];Zhang, Weian[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Sch Chem & Mol Engn, Meilong Rd 130, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Shanghai Key Lab Orbital Dis & Ocular Oncol, Dept Ophthalmol, Peoples Hosp 9,Sch Med, Zhizaoju Rd 639, Shanghai 200011, Peoples R China

年份：2019

卷号：7

期号：8

起止页码：3266

外文期刊名：BIOMATERIALS SCIENCE

收录：;EI(收录号：20193007237678);WOS:【SCI-EXPANDED(收录号:WOS:000476955100015)】；

基金：This work was financially supported by the National Natural Science Foundation of China (21574039 and 21875063), and the Science and Technology Commission of Shanghai Municipality (15JC1401402 and 17DZ2260100).

语种：英文

外文关键词：Stem cells - Enzyme activity - Crosslinking - Biodegradation - Bone - Chitosan - Energy dissipation - Tissue regeneration - Cell engineering - Cell culture - Biodegradability

摘要：Stem cell therapy based on advanced biomaterials provides a promising strategy in bone tissue engineering. Nevertheless, guided bone regeneration which fulfills the criteria in terms of biomechanics, biodegradability and bioactivity is highly appealing but challenging. Inspired by the superior double-network (DN) structure, herein, a biodegradable hybrid DN hydrogel is proposed to promote in situ bone regeneration. The DN hydrogel is constructed by interspersing a methacrylated gelatin (GelMA) network into a well-defined nanocomposite (NC) hydrogel consisting of methacrylated chitosan (CSMA) and polyhedral oligomeric silsesquioxane (POSS) via a two-step photo-crosslinking process. The hybrid DN hydrogel has the following advantageous characteristics: (i) it exhibits enhanced stiffness and toughness benefiting from the inorganic POSS units and unique energy dissipation; (ii) naturally occurring biomacromolecules (chitosan and gelatin) as the hydrogel framework result in an appropriate biodegradation behavior, which can be replaced by newly formed tissues; (iii) it preferentially guides mesenchymal stem cells (MSCs) toward osteogenic differentiation in vitro by detecting the elevated levels of enzyme activity and calcium deposition along with the up-regulated osteogenesis-related genes and proteins; and (iv) accelerated in situ bone regeneration is observed after implanting MSC-loaded hydrogels into rat calvarial defects. Therefore, we provide a new insight to develop functional hydrogels for triggering specific cellular responses toward stem cell therapy and bone-related tissue engineering.