文献类型：期刊文献

英文题名：Optimizing the chemical recognition process of a fluorescent chemosensor for α-ketoglutarate

作者：He, Ye[1]; Guo, Zhiqian[1]; Jin, Pengwei[1]; Jiao, Changhong[1]; Tian, He[1]; Zhu, Weihong[1]

机构：[1] Key Laboratory for Advanced Materials and Institute of Fine Chemicals, Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai, China

年份：2015

卷号：54

期号：11

起止页码：2886

外文期刊名：Industrial and Engineering Chemistry Research

收录：EI(收录号：20151300695182);Scopus(收录号：2-s2.0-84925814042)

语种：英文

外文关键词：Body fluids - Diagnosis - Fluorescence - Functional groups - Physiology - Reaction kinetics

摘要：α-Ketoglutarate (α-KA) can convert to 2-hydroxyglutarate (2-HG), which is confirmed to be associated with many diseases, especially with acute myeloid leukemia (AML). In this paper, a novel reaction-based chemosensor DT based on the typical Schiff-base reaction was designed for sensing the biomarker of α-KA, in which a diazanyl group as the recognition group was linked with a benzothiadiazole unit as the fluorophore moiety. Considering the typical Schiff-base reaction to generate hydrazones suffering from slow kinetics, particularly under neutral conditions, a series of parallel experiments was conducted for optimizing the chemical recognition process, including varying the solvent, reaction temperature, reactant concentration, and reaction rate. The optimum condition was established as a pH value, temperature, α-KA concentration, and response time of 5.7, 30 °C, 100 μM, and 20 min, respectively. Notably, in contrast with the initial 6.3-fold fluorescence enhancement, the remarkable 75-fold fluorescence enhancement ((I-I0)/I0at 560 nm) was observed by optimizing the chemical recognition process of DT and α-KA. Finally, DT was carried out for the chemical recognition processing of α-KA in serum. We demonstrated that DT is selective for α-KA over other potential biologically interferences with similar structures and thus is suitable for detecting α-KA in serum. On the basis of the optimized chemical recognition process, DT shows high potential application for sensing α-KA with remarkable fluorescence enhancement. This work provided a potential method that is quick and convenient for sensing biomarker α-KA in serum. It is worth noting that without complicated pretreatment, utilizing a novel reaction-based fluorescent chemosensor may establish a new promising platform for clinical diagnosis biomarker. ? 2015 American Chemical Society.