文献类型：期刊文献

英文题名：Sensitive detection and labelling of proteins with a molecular rotor-based fluorogenic probe

作者：Chen, Na[1];Wu, Qingyi[1];Yuan, Xia[1];Xie, Hexin[1]

机构：[1]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Pharm, State Key Lab Bioreactor Engn,Shanghai Key Lab New, Shanghai 200237, Peoples R China

年份：2025

卷号：23

期号：24

起止页码：5773

外文期刊名：ORGANIC & BIOMOLECULAR CHEMISTRY

收录：;EI(收录号：20252218516134);WOS:【SCI-EXPANDED(收录号:WOS:001497244800001)】；

基金：The authors acknowledge funding support from the NSFC (22277027), the Fundamental Research Funds for the Central Universities, and Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism (Shanghai Municipal Education Commission, grant 2021 Sci & Tech 03-28).

语种：英文

摘要：Reported herein is a sensitive fluorogenic reagent based on an environment-sensitive molecular rotor for detecting and labelling proteins. The rapid binding of the probe to protein amino residues results in a significant enhancement in fluorescence emission, while its reversible interaction with free amino acids remains non-fluorescent. We demonstrate the utility of this reagent for wash-free and real-time imaging of membrane proteins in living cells and for rapidly determining the minimal inhibitory concentration (MIC) of antibiotics against bacteria.