文献类型：期刊文献

英文题名：Embryogenic callus induction, cell suspension culture, and spectrum-effect relationship between antioxidant activity and polyphenols composition of Siraitia grosvenorii cultured cells

作者：Liu, Zebo[1];Zhu, Xiaofeng[1];Mohsin, Ali[1];Yin, Zhongping[2];Zhuang, Yingping[1];Zhou, Bei[1];Du, Linxiao[1];Yin, Xuejian[1];Liu, Nan[1];Wang, Zejian[1];Guo, Meijin[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Jiangxi Agr Univ, Jiangxi Key Lab Nat Prod & Funct Foods, Nanchang 330045, Jiangxi, Peoples R China

年份：2022

卷号：176

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20215111362969);WOS:【SCI-EXPANDED(收录号:WOS:000736973500006)】；

基金：We appreciate the financial support of Layn Biotech Co. Ltd. (Guilin, China).

语种：英文

外文关键词：Siraitia grosvenorii cultured cells; Polyphenols; Response surface methodology; Antioxidation; Spectrum-effect relationship

摘要：Siraitia grosvenorii (S. grosvenorii), a perennial vine of Cucurbitaceae family, has been widely used in medicine and food products. However, its application is hindered due to the limited output of natural and cultivated plants. Thus, to expand plant resource and application of S. grosvenorii, the current research study comprises embryogenic callus induction, development of suspension culture, and analysis on the spectrum-effect relationship between antioxidant activity and polyphenols composition of S. grosvenorii cultured cells. Adding 0.5 g.L- 1 ascorbic acid and 1.0 g.L- 1 choline could increase the induction rate from 0.75% to 68% and enhances the antioxidant enzyme activities of loose embryogenic calluses. A total of 15 polyphenols (14 flavonols and 1 phenolic acid) in the callus extracts were identified via liquid chromatography-mass spectrometry. Through Plackett-Burman's design and response surface methodology, optimal proportionings of plant growth regulator (PGRs) for cell suspension culture were obtained. With the optimized PGRs, the obtained cell biomass and polyphenols content were 32.18% and 13.86%, respectively, more than PGRs proportioning before optimization. Furthermore, a homogeneous and rapidly grown suspension cell culture was developed with a maximum biomass and polyphenols content of 25.5 g.L- 1 DW and 30.75 mg.g- 1 DW, respectively. The results of spectrum-effect relationships analysis indicated a strong linear correlation between 15 above-identified polyphenols and the antioxidation activity of the extracts. In addition, the partial least square method result revealed that all 15 polyphenols positively contributed to antioxidant capacity, including 3,4-di-p-coumaroyl, 5-tri-hydroxycoumaroyl quinic acid and kaempferol-3-O-Glc-7-O-Rha with vital roles. Our results indicated that the extracts from S. grosvenorii cell have excellent antioxidant activity, and therefore have potential applications in healthy food and daily cosmetics.