文献类型：期刊文献

英文题名：Click dipeptide: A novel stationary phase applied in two-dimensional liquid chromatography

作者：Xue, Meiyun[1];Huang, Hongxue[1];Ke, Yanxiong[1];Chu, Changhu[1];Jin, Yu[1];Liang, Xinmiao[1,2]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Minist Educ, Engn Res Ctr Pharmaceut Proc Chem, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

年份：2009

卷号：1216

期号：49

起止页码：8623

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;EI(收录号：20094612451314);WOS:【SCI-EXPANDED(收录号:WOS:000272089200008)】；

基金：This work was sponsored by the start-up fund and 111 Project (Grant No. 807023) from East China University of Science and Technology, National High Technology Research and Development Program of China (863 Program, No. 2008AA06Z306) and National Natural Science Fund for Distinguished Young Scholars (20825518).

语种：英文

外文关键词：Click dipeptide stationary phase; alpha-Azido L-phenylglycine dipeptide; Orthogonality; 2D-RP/RPLC; Complex sample

摘要：2D-HPLC is an important technique for the separation of complex samples. Developing new types of stationary phases is of great interest to construct 2D-LC systems with high orthogonality. In this study, a novel stationary phase-Click dipeptide (L-phenylglycine dipeptide) was prepared by immobilization of alpha-azido L-phenylglycine dipeptide on alkyne-silica via click chemistry. In the preparation of this new material, an efficient, inexpensive and shelf-stable diazo transfer reagent (imidazole-1-sulfonyl azide hydrochloride) was utilized to transfer the amino group of L-phenylglycine to corresponding azido group under mild conditions. The Click dipeptide thus prepared was confirmed by FT-IR, solid state CP/MAS C-13 NMR and elemental analysis. The Click dipeptide packing showed high orthogonality with C18, which reached 63.5%. An off-line 2D-RP/RPLC system was developed to analyze a traditional Chinese medicine (TCM)-Rheum Palmatum L. The results showed high orthogonality between Click dipeptide and C18 as well as great separating power in the practical separation of complex samples. (C) 2009 Elsevier B.V. All rights reserved.