文献类型：期刊文献

英文题名：Preparative isolation of ganoderic acid S, ganoderic acid T and ganoderol B from Ganoderma lucidum mycelia by high-speed counter-current chromatography

作者：Feng, Na[1,2,3];Wei, Yutian[4];Feng, Jie[1,2,3];Tang, Qingjiu[1,2,3];Zhang, Zhong[1,2,3];Zhang, Jingsong[1,2,3];Han, Wei[4]

机构：[1]Natl Engn Res Ctr Edible Fungi, Minist Agr, Shanghai, Peoples R China;[2]Shanghai Acad Agr Sci, Inst Edible Fungi, Natl R&D Ctr Edible Fungi Proc, Shanghai, Peoples R China;[3]Key Lab Edible Fungi Resources & Utilizat South, Shanghai, Peoples R China;[4]East China Univ Sci & Technol, Sch Pharm, Shanghai, Peoples R China

年份：2018

卷号：32

期号：10

外文期刊名：BIOMEDICAL CHROMATOGRAPHY

收录：;EI(收录号：20232414241772);WOS:【SCI-EXPANDED(收录号:WOS:000445175600001)】；

基金：Key Technology and Application of Ganoderma Trieterpenes Preparation, Grant/Award Number: No. 17391903300

语种：英文

外文关键词：bioactive triterpenes; Ganoderma lucidum; high-performance liquid chromatography; high-speed counter-current chromatography

摘要：Ganoderic acid S, ganoderic acid T and ganoderal B are the main bioactive triterpenes of Ganoderma lucidum. In this study, mycelia of G. lucidum were obtained by two-stage fermentation and then extracted by ethanol and petroleum ether sequentially to obtain crude triterpenes. The crude sample was further purified by recycling high-speed counter-current chromatography with n-hexane-ethyl acetate-methanol-water (7:12:11:5, v/v/v/v) as the optimized two-phase solvent system. A 16.4 mg aliquot of ganoderol B with a purity of 90.4% was separated from 300 mg of the crude sample in a single run. After employing the recycling elution mode of HSCCC with n-hexane-ethyl acetate-methanol-water (6:10:8:4.5, v/v/v/v) for five cycles, 25.7 mg ganoderic acid T and 3.7 mg ganoderic acid S with purities of 97.8 and 83.0%, respectively, were obtained. The purities of three compounds were determined by high-performance liquid chromatography and their chemical structures were identified by NMR and MS data.