文献类型：期刊文献

英文题名：Effects of light/dark cycle, mixing pattern and partial pressure of H₂ on biohydrogen production by Rhodobacter sphaeroides ZX-5

作者：Li, Xu[1];Wang, Yonghong[1];Zhang, Siliang[1];Chu, Ju[1];Zhang, Ming[1];Huang, Mingzhi[1];Zhuang, Yingping[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2011

卷号：102

期号：2

起止页码：1142

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000286782700099)】；

基金：This study was financially supported by National Basic Research Program of China (973 Program, 2007CB714303), the National Special Fund for State Key Laboratory of Bioreactor Engineering (2060204) and Shanghai Leading Academic Discipline Project (B505).

语种：英文

外文关键词：Photo-hydrogen production; Rhodobacter sphaeroides; Light/dark cycle; Shaking velocity; Hydrogen partial pressure

摘要：The effects of light/dark cycle, mixing pattern and partial pressure of H-2 on the growth and hydrogen production of Rhodobacter sphaeroides ZX-5 were investigated. The results from light/dark cycle culture showed that little or no hydrogen production was observed during the dark periods, and the hydrogen production immediately recovered once illumination was resumed. Also, it was found that the optimum condition of shaking velocity was 120 rpm for hydrogen photo-fermentation. Meanwhile, shaking during H-2 production phase (i.e., cell growth stationary phase) of photo-fermentation played a crucial role on effectively enhancing the phototrophic hydrogen production, rather than that during cell exponential growth phase. The other factor evaluated was hydrogen partial pressure in the culture system. The substrate conversion efficiency increased from 86.07% to 95.56% along with the decrease of the total pressure in the photobioreactor from 1.082 x 10(5) to 0.944 x 10(5) Pa, which indicated that reduction of H-2 partial pressure by lowering the operating pressure substantially improved H-2 production in an anaerobic, photo-fermentation process. (C) 2010 Elsevier Ltd. All rights reserved.