文献类型：期刊文献

英文题名：Delivery of Encapsulated Intelligent Engineered Probiotic for Inflammatory Bowel Disease Therapy

作者：Zou, Zhen-Ping[1];Cai, Zhihao[1,2];Zhang, Xiao-Peng[1];Zhang, Donghui[1];Xu, Chu-Ying[3];Zhou, Ying[1];Liu, Runhui[1,2];Ye, Bang-Ce[1,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Frontiers Sci Optogenet Techn Cell, Key Lab Ultrafine Mat, Frontiers Sci Mat & Dynam Chem,Minist Educ,Sch Ma, Shanghai 200237, Peoples R China;[3]Zhejiang Univ Technol, Inst Engn Biol & Hlth, Collaborat Innovat Ctr Yangtze River Delta Reg G, Coll Pharmaceut Sci, Hangzhou 310014, Zhejiang, Peoples R China

年份：2025

卷号：14

期号：3

外文期刊名：ADVANCED HEALTHCARE MATERIALS

收录：;EI(收录号：20244917485370);WOS:【SCI-EXPANDED(收录号:WOS:001369526500001)】；

基金：This work was sponsored by the National Key Research and Development Program of China (2023YFF1204500, 2022YFC2303100), the National Natural Science Foundation of China (22205063, 22134003, T2325010, 22075078), China Postdoctoral Science Foundation (2023M741176, 2024T170274), Shanghai Sailing Program (22YF1410100), Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism (Shanghai Municipal Education Commission). The authors thank Research Center of Analysis and Test of East China University of Science and Technology for the help on the characterization.

语种：英文

外文关键词：inflammatory bowel disease; intelligent engineered probiotic; intestinal delivery system; mucus encapsulated microsphere gel; polyserine modified alginates

摘要：Engineered bacterial therapy holds enormous potential for treating intestinal diseases, employing synthetic biology techniques to achieve localized drug delivery within intestines. However, effective delivery of engineered bacteria to lesion sites and ensuring sustained colonization remain challenging. Here, a mucus encapsulated microsphere gel (MM) delivery system is developed to encapsulate genetically engineered bacteria capable of detecting and treating enteritis. The MM delivery system features an external mucosal coating composed of hyaluronic acid and epigallocatechin gallate, along with internal microspheres of highly biocompatible polyserine modified alginates encapsulating with the engineered probiotics. The MM delivery system effectively protects engineered bacteria harsh environment in stomach and significantly improves intestinal adhesion of the probiotics, extending colonization up to 24 h, and does not affect the entry of biomarker or release of Avcystatin. It exhibits notable diagnostic and therapeutic efficacy in inflammatory bowel disease models, thus facilitating the advancement of live biotherapeutic products toward clinical application.