文献类型：期刊文献

中文题名：巨大芽孢杆菌发酵合成有机酸的条件优化

英文题名：Optimization of organic acid production by Bacillus megaterium fermentation

作者：王心语[1];余君雄[2];张子恕[2];肖慈英[2];张红兵[1];王泽建[2]

机构：[1]河北经贸大学生物科学与工程学院,河北石家庄050061;[2]华东理工大学生物工程学院,上海200237

年份：2026

卷号：47

期号：3

起止页码：274

中文期刊名：河北科技大学学报

外文期刊名：Journal of Hebei University of Science and Technology

收录：;北大核心:【北大核心2023】；

基金：京津冀环境综合治理国家科技重大专项(2025ZD1203400)。

语种：中文

中文关键词：发酵工程;巨大芽孢杆菌;有机酸;工艺优化;过程控制

外文关键词：fermentation engineering;Bacillus megaterium;organic acids;process optimization;process control

摘要：为提高巨大芽孢杆菌(Bacillus megaterium)补料分批发酵体系的有机酸产量与底物转化率,采用摇瓶与5 L生物反应器,对培养基组分和发酵过程控制工艺条件进行优化,并建立在线耗氧速率(oxygen uptake rate,OUR)指导的发酵过程供氧调控策略及适宜的pH调节剂模式。结果表明:影响有机酸产量与底物转化率最主要的因素为碳源、MnSO_(4)·H_(2)O、pH值、供氧水平及pH调节剂组成;最佳发酵条件为以葡萄糖为碳源,添加0.1 g/L MnSO_(4)·H_(2)O,在5 L发酵罐内控制pH为5.5,采用OUR反馈进行适度供氧,使用体积比为8∶2的NaOH-氨水混合溶液作为pH调节剂;应用该优化配方及工艺,巨大芽孢杆菌发酵的有机酸最高产量达到165.61 g/L,糖酸转化率达到50.41%。研究结果能够高效提升有机酸生产效率和底物转化率,为工业化放大生产提供了实验依据。
To improve the organic acid yield and substrate conversion efficiency in the fed-batch fermentation system of Bacillus megaterium,this study optimized the medium components and fermentation process control conditions using shake flasks and a 5 L bioreactor.An oxygen supply control strategy was guided by the quantitative oxygen uptake rate(OUR)and an optimal pH regulator composition was established for the fermentation process.The results show that the main factors affecting organic acid yield and substrate conversion efficiency are carbon source,MnSO_(4)·H_(2)O,pH value,oxygen supply level,and pH regulator composition.The optimal fermentation conditions are determined as follows:glucose as the carbon source,addition of 0.1 g/L MnSO_(4)·H_(2)O,pH controlled at 5.5 in a 5 L fermenter,moderate oxygen supply regulated by OUR feedback,and a mixture of NaOH and ammonia water at a ratio of 8∶2 as the pH regulator.Under these optimized conditions,the maximum organic acid yield reaches 165.61 g/L,and the glucose conversion rate reaches 50.41%.These results can effectively enhance the organic acid production efficiency and substrate conversion rate,providing reliable technical support for industrial scale-up production.