文献类型：期刊文献

中文题名：SAM产生菌酿酒酵母HYS98发酵动力学及比生长速率控制策略

英文题名：Kinetics and Specific Growth Rate Control Strategy of Saccharomyces cerevisiae HYS98 Fermentation for High Productivity of SAM

作者：张昀羿[1];李元广[1];金建[1];杨东[2];沈国敏[1]

机构：[1]华东理工大学海洋生化工程研究所,生物反应器工程国家重点实验室,上海200237;[2]上海泽元海洋生物技术有限公司,上海201203

年份：2005

卷号：5

期号：3

起止页码：322

中文期刊名：过程工程学报

外文期刊名：The Chinese Journal of Process Engineering

收录：CSTPCD;;EI(收录号：2005279197579);Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家十五科技攻关计划资助项目(编号:2004BA713B01-07)

语种：中文

中文关键词：S-腺苷甲硫氨酸;恒化培养;动力学模型;流加培养

外文关键词：S-adenosyl-L-methionine; chemostat cultivation; kinetic model; fed-batch cultivation

摘要：通过恒化培养对S-腺苷甲硫氨酸(SAM)产生菌酿酒酵母HYS98发酵动力学进行了研究.结果表明,该菌株的生长与限制性基质蔗糖浓度之间符合Monod关系式,最大比生长速率μmax为0.28h-1,饱和常数KS为1.27g/L;当0.005h?1≤μ≤0.11h-1,产物生成模型为qSAM=-1.39μ2+0.19μ,底物消耗动力学模型为qS=μ/0.62+0.012.基于建立的动力学模型,提出了分阶段控制菌体比生长速率的流加培养策略,据此策略在5L反应器中发酵24h,SAM浓度达3.56g/L,而SAM产率达0.15g/(L?h),比文献报道的最高水平高67%.
The fermentation kinetics of S-adenosyl-L-methionine (SAM) producing Saccharomyces cerevisiae HYS98 was studied through chemostat cultivation. The results showed that the relationship of biomass specific growth rate (μ) and residual sucrose concentration was in accordance with the Monod equation μ=μmaxCS/(KS+CS), where μmax=0.28 h?1, KS=1.27 g/L. The production of SAM followed the mathematical equation qSAM=?1.39μ2+0.19μ, when 0.005 h?1≤μ≤0.11 h?1. The sucrose consumption kinetics followed the equation qS=μ/0.62+0.012. To maximize the SAM productivity, a two-stage process control strategy was proposed based on the kinetic studies. By applying the strategy to the cultivation in a 5 L bioreactor, the accumulation of SAM reached 3.56 g/L and its production rate reached 0.15 g/(L?h) in 24 h, which was 67% increase over the highest level reported so far.