文献类型：期刊文献

英文题名：Discovery and characterization of two novel polyethylene terephthalate hydrolases: One from a bacterium identified in human feces and one from the Streptomyces genus

作者：Han, Zhengyang[1];Zhang, Xiaoyan[1];Huang, Hanyao[1];Fan, Daidi[2];Bai, Yunpeng[1]

机构：[1]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Northwest Univ, Shaanxi R&D Ctr Biomat & Fermentat Engn, Sch Chem Engn, Xian 710069, Shaanxi, Peoples R China

年份：2024

卷号：472

外文期刊名：JOURNAL OF HAZARDOUS MATERIALS

收录：;EI(收录号：20242016088325);WOS:【SCI-EXPANDED(收录号:WOS:001241455100001)】；

基金：Acknowledgments This research was supported by the National Key Research and Development Program of China (2023YFA0913603, 2021YFC2102804) and the National Natural Science Foundation of China (No. 22378120, 22078096) .

语种：英文

外文关键词：Polyethylene terephthalate; Plastic degradation; PET hydrolase; Pseudomonas; Streptomyces

摘要：Polyethylene terephthalate (PET) is widely used for various industrial applications. However, owing to its extremely slow breakdown rate, PET accumulates as plastic trash, which negatively affects the environment and human health. Here, we report two novel PET hydrolases: Pp PETase from Pseudomonas paralcaligenes MRCP1333, identified in human feces, and Sc PETase from Streptomyces calvus DSM 41452. These two enzymes can decompose various PET materials, including semicrystalline PET powders (Cry -PET) and low -crystallinity PET films (gfPET). By structure -guided engineering, two variants, Pp PETase Y239R/F244G/Y250G and Sc PETase A212C/T249C/N195H/ N243K were obtained that decompose Cry -PET 3.1- and 1.9 -fold faster than their wild -type enzymes, respectively. The co -expression of Sc PETase and mono-(2-hydroxyethyl) terephthalate hydrolase from Ideonella sakaiensis ( Is MHETase) resulted in 1.4 -fold more degradation than the single enzyme system. This engineered strain degraded Cry -PET and gf-PET by more than 40% and 6%, respectively, after 30 d. The concentrations of terephthalic acid (TPA) in the Cry -PET and gf-PET degradation products were 37.7% and 25.6%, respectively. The discovery of these two novel PET hydrolases provides opportunities to create more powerful biocatalysts for PET biodegradation.