文献类型：期刊文献

英文题名：Photochromic Fluorescent Probe Strategy for the Super-resolution Imaging of Biologically Important Biomarkers

作者：Chai, Xianzhi[1,2];Han, Hai-Hao[1,2,3];Sedgwick, Adam C.[4];Li, Na[5];Zang, Yi[3];James, Tony D.[6];Zhang, Junji[1,2];Hu, Xi-Le[1,2];Yu, Yang[5];Li, Yao[5];Wang, Yan[5];Li, Jia[3];He, Xiao-Peng[1,2];Tian, He[1,2]

机构：[1]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Joint Int Res Lab Precis Chem, Shanghai 200237, Peoples R China;[3]Chinese Acad Sci, Natl Ctr Drug Screening, Shanghai Inst Mat Med, State Key Lab Drug Res, 189 Guo Shoujing Rd, Shanghai 201203, Peoples R China;[4]Univ Texas Austin, Dept Chem, Austin, TX 78712 USA;[5]Chinese Acad Sci, Natl Ctr Prot Sci Shanghai, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China;[6]Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England

年份：2020

卷号：142

期号：42

起止页码：18005

外文期刊名：JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

收录：;EI(收录号：20204909573835);WOS:【SCI-EXPANDED(收录号:WOS:000580559000024)】；

基金：This work was supported by the NSFC (21788102, 91853201, 21878086, and 21722801), the Shanghai Municipal Science and Technology Major Project (2018SHZDZX03), the National Key Sci-Tech Special Projects of Infection Diseases of China (2018ZX10732202), the International Cooperation Program of the Shanghai Science and Technology Committee (17520750100), and the Shanghai Rising-Star Program (19QA1402500 to J.Z.). The authors acknowledge Jie Gao and Ying Dong for help with cell culturing. T.D.J. thanks the Royal Society for a Wolfson Research Merit Award.

语种：英文

外文关键词：Stochastic systems - Photochromism - Probes - Fluorescence spectroscopy - Cell culture - Fluorescence - Storms

摘要：Here, we report a beta-galactosidase (beta-Gal)-responsive photochromic fluorescent probe, NpG, that was designed to prebind to human serum albumin (HSA) to form the probe/protein hybrid, NpG@HSA. The formation of NpG@HSA led to an increase in fluorescence emission (520 nm) corresponding to the binding of the fluorescent naphthalimide unit with HSA. In addition, this enabled visualization of the spiropyran fluorescence emission in aqueous media. Our probe/protein hybrid approach afforded a unique imaging platform with enhanced cell permeability and solubility that was capable of visualizing the cellular uptake of NpG@HSA before its activation by beta-Gal. The beta-Gal-mediated cleavage of the galactose unit within the NpG@HSA hybrid resulted in the formation of NpM@HSA and an increase in red fluorescence emission (620 nm). The resultant merocyanine unit was then able to undergo photoisomerization (merocyanine <-> spiropyran) to facilitate STORM (i.e., stochastic optical reconstruction microscopy) imaging with minimal phototoxicity and excellent photostability/reversibility. Using STORM, NpG@HSA was able to determine the subcellular distribution of beta-Gal activity between cell lines with nanoscale precision. We believe that this system represents a versatile imaging platform for the design of photochromic fluorescent probes suitable for illuminating the precise location of disease-specific biomarkers in various cellular processes.