文献类型：期刊文献

英文题名：Utilizing cationic liposome to co-deliver poly-siRNA and chlorantraniliprole complexes: A new strategy for overcoming insecticide resistance in Plutella xylostella (Lepidoptera: Plutellidae)

作者：Xu, ZhiPing[1];Wang, XinQian[1];Shao, Xusheng[1];Yang, YangYang[1];Zhu, Kun Yan[2]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA

年份：2026

卷号：5

期号：2

起止页码：108

外文期刊名：ADVANCED AGROCHEM

收录：WOS:【ESCI(收录号:WOS:001802721700001)】；

基金：Our research was supported by the Shanghai Agricultural Science and Technology Innovation Program (Grant No. K2025008) .

语种：英文

外文关键词：Rolling circle transcription; Plutella xylostella; RNA interference; Co-delivery; Resistance management

摘要：The diamondback moth (Plutella xylostella) is a widely distributed pest recognized for its damaging effects on cruciferous crops and its rapid development of resistance to insecticides, particularly chlorantraniliprole (CAP). This study aims to develop new strategies for managing CAP resistance through the co-delivery of CAP and polysiRNA targeting cytochrome P450 (CYP) genes. Utilizing a cationic liposome (CTL), we developed a unique codelivery system for CTL-CAP/poly-siRNA-CYP complexes, characterized by an average particle size of 705 nm, a surface charge of +25 mV, and an impressive polydispersity index (PDI) of 0.12, demonstrating good formulation quality. Feeding bioassays revealed a remarkable capacity of these complexes to suppress CYP genes (CYP6BG1 and CYP6BF1v4) expression in both the moderately CAP-resistant (CAP-SJ) and highly resistant (CAP-QP) P. xylostella, resulting in an about 30 % enhancement in CAP efficacy compared to the control group. This synergistic approach is among the first to combine RNAi and insecticides to combat resistance in this pest. This groundbreaking research not only introduces an innovative method for integrating RNAi-mediated gene silencing with chemical insecticides, but also significantly improves the effectiveness of CAP against resistant P. xylostella populations.