文献类型：期刊文献

英文题名：Non-invasive Video Image-based Analysis Method Coupled to Field Potential Recording for Evaluation of the Drug-induced Effect in Cardiac Tissue

作者：Espulgar, Wilfred Villariza[1];Saito, Masato[1];Lee, Jong-Kook[2];Yamaguchi, Yoshinori[1,3];Tamiya, Eiichi[1]

机构：[1]Osaka Univ, Grad Sch Engn, Dept Appl Phys, 2-1 Yamadaoka, Suita, Osaka 5650871, Japan;[2]Osaka Univ, Grad Sch Med, Dept Cardiovasc Regenerat Med, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan;[3]E China Univ Sci & Technol, IPBM, Grad Sch Sci, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2016

卷号：84

期号：5

起止页码：283

外文期刊名：ELECTROCHEMISTRY

收录：;EI(收录号：20162002381423);WOS:【SCI-EXPANDED(收录号:WOS:000375892700003)】；

语种：英文

外文关键词：Field Potential; Drug Screening; Video Analysis; Cardiac Tissue

摘要：Reports on the use multi electrode arrays (MEA) systems for screening the electrophysiological effects of drug candidates on cardiac tissues as a response to the need of the pharmaceutical industry for a high-throughput screening method have increased in the past decades. Though the field potential (FP) measured in MEA have been reported coherent to the progression of the well-established progression of action potential (AP), certain pharmacological effects do not accurately reflect in the observed FP waveform alteration. Here, we revisit the imaging analysis algorithm developed in our lab based on the pixel intensity variation that can be utilized to improve the FP measurement and interpretation. Imaging analysis was done on videos recorded with a microscope camera at 30 Hz frame rate and 680 x 510 pixel resolution resulting to a relative lower system requirement compared to other methods. It is expected that combining our method with MEA systems can greatly aid in developing a screening platform for cardiovascular effect profiling of candidate drugs. (C) The Electrochemical Society of Japan, All rights reserved.