文献类型：期刊文献

英文题名：In situ ornamenting poly(ε-caprolactone) electrospun fibers with different fiber diameters using chondrocyte-derived extracellular matrix for chondrogenesis of mesenchymal stem cells

作者：Xu, Jiazhu[1];Fang, Qi[1];Liu, Yanli[1];Zhou, Yan[1];Ye, Zhaoyang[1];Tan, Wen-Song[1]

机构：[1]East China Univ Sci & Technol, State Kay Lab Bioreactor Engn, 130 Mei Long Rd,POB 309, Shanghai 200237, Peoples R China

年份：2021

卷号：197

外文期刊名：COLLOIDS AND SURFACES B-BIOINTERFACES

收录：;EI(收录号：20204109307558);WOS:【SCI-EXPANDED(收录号:WOS:000603351700003)】；

基金：This work was supported by the National Key Research and Development Program of China (Grant No. 2018YFC1105800), the National Natural Science Foundation of China (Grant No. 81671841), and the Fundamental Research Funds for the Central Universities (Grant No. 22221818014).

语种：英文

外文关键词：Electrospinning; Articular chondrocytes; Decellularization; Chondrogenesis; Mesenchymal stem cells

摘要：Biomimetic instructive tissue engineering scaffolds are critical for achieving successful tissue regeneration. In the present study, we developed a novel scaffold via ornamenting poly(epsilon-caprolactone) (PCL) electrospun fibers with a chondrocyte-derived extracellular matrix (ECM)-coating, which was applied for chondrogenesis of mesenchymal stem cells (MSCs). PCL fibrous films with different fiber diameters (1282 +/- 121 nm, 549 +/- 61 nm and 285 +/- 38 nm) were first prepared via electrospinning. Rabbit articular chondrocytes (rACs) were cultured on PCL fibrous scaffolds, followed by a decellularization treatment to generate decellularized ECM (dECM)-coated PCL scaffolds (dECM/PCL). Rabbit bone marrow-derived MSCs (rMSCs) were then seeded onto these scaffolds and adhesion, proliferation and chondrogenic differentiation were evaluated. dECM/PCL scaffolds displayed distinct surface microstructural features with varying fiber diameters and fibrous mesh-like ECM with more developed collagen fibers was observed on nanofibers. On dECM/PCL scaffolds, rMSCs tended to spread more at 24 h post-seeding and proliferated better within 7 d compared to those on uncoated PCL scaffolds. Based on analysis of gene expression, rMSCs underwent the best chondrogenic differentiation on dECM/PCL scaffolds of 549-nm fibers. Collectively, such dECM/PCL composite scaffolds are very promising for cartilage tissue regeneration.