文献类型：期刊文献

中文题名：壳二糖、壳三糖单体制备及其结构分析

英文题名：Monomer preparation and structure analysis of chitobiose and chitotriose

作者：徐庆[1];陈列欢[2];秦臻[1];陈启明[1];邱勇隽[1];赵黎明[1]

机构：[1]华东理工大学生物反应器国家重点实验室,食品科学与工程系,上海200237;[2]惠州长龙生物技术有限公司,广东惠州516000

年份：2017

卷号：38

期号：13

起止页码：13

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2017_2018】；

基金：国家自然基金项目(31371725);上海市曙光计划(15SG28)

语种：中文

中文关键词：壳聚糖;酶解;离子交换色谱法;壳二糖;壳三糖

外文关键词：chitosan ; enzymatic hydrolysis ; ion exchange chromatography ; chitobiose ; chitotriose

摘要：壳寡糖因自身结构特征而存在多种生物活性,目前对壳寡糖的活性研究大部分是不同聚合度的混合物。为获得单一聚合度壳二糖、壳三糖,采用了专一性壳聚糖酶对壳聚糖进行降解,运用薄层色谱法(TLC)判断酶解反应终点,并采用高效液相色谱法(HPLC)法进行定量分析。结果表明酶解组分主要为壳二糖、壳三糖,含量分别为65.63%,32.48%。将酶解后的混合组分采用实验室自制的阳离子交换树脂QY-H003对酶解产物进行分离,以盐酸浓度为C_1=1.25 mol/L、C_2=1.55 mol/L为洗脱剂进行梯度洗脱得到两个组分。采用TLC、HPLC、红外光谱(FT-IR)及核磁共振氢谱(1HNMR)检测手段对组分进行分析。分离纯化获得的壳二糖、壳三糖组分经HPLC法检测,纯度分别98.06%,96.00%。壳二糖回收率达92.46%、壳三糖回收率达95.53%。该制备工艺放大后可实现高纯度壳二糖、壳三糖单体规模化制备,为壳二糖、壳三糖的生理活性研究及应用提供物质基础。
Chitooligosaccharides has a variety of bioactivities due to its special structure features. Currently, almost all of chitooligosaceharides used in activity research were mixtures of polymers with different degrees of polymerization （DP）.In this study, the specific ehitosan enzyme was used to degrade chitosan to prepare ehitobiose and chitotriose. The TLC analysis was then used to determine the end point of enzymatic hydrolysis reaction, and the component of hydrolysis products were analyzed by HPLC.The results showed that the enzymatic hydrolysates consisted of chitobiose （65.63 % ）and ehitotriose （32.48 % ）.Then, chitobiose and chitotriose were separated by a home- made cation ion exchange resin （ QY- H003 ） with hydrochloric acid solutions at different concentrations（ C1= 1.25 mol/L, C2 = 1.55 mol/L） .Further analysis was developed by TLC, HPLC, FT-IR and ^1HNMR.The purity of separated chitobiose and chitotriose were 98.06% and 96.00% ,respectively.The recovery rate was 92.46% and 95.53% ,respectively. It indicates that the preparation process was suited for scale production of chitobiose and chitotriose with high purity, and it benefits for the future bioaetivity studies and applications of chitooligosaccharides.