文献类型：期刊文献

英文题名：Biocatalytic resolution of glycidyl aryl ethers by Trichosporon loubierii:: cell/substrate ratio influences the optical purity of (R)-epoxides

作者：Xu, Y; Xu, JH; Pan, J; Tang, YF

机构：[1]E China Univ Sci & Technol, Lab Biocatalysis & Bioproc, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2004

卷号：26

期号：15

起止页码：1217

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000223044800007)】；

语种：英文

外文关键词：cell/substrate ratio; enantioselective hydrolysis; epoxide hydrolase; glycidyl aryl ether; Trichosporon loubierii

摘要：Glycidyl aryl ethers (1a-1e) were resolved by using lyophilized cells of Trichosporon loubierii ECU1040 having epoxide hydrolase activity. The activity and enantioselectivity depended on the structure of the aryl group. Different cell/substrate ratios also influenced the optical purity of remaining substrate. An additional stability test of the whole-cell enzyme suggests that rapid deactivation of the epoxide hydrolase was the potential reason. (R)-Epoxides were prepared in gram amounts with optical purity of 87%-99% ee.