文献类型：期刊文献

英文题名：The immunomodulatory role of sulfated chitosan in BMP-2-mediated bone regeneration

作者：Shu, Yang[1,2];Yu, Yuanman[1,2];Zhang, Shuang[1,2];Wang, Jing[1,2];Xiao, Yin[3];Liu, Changsheng[1,2,4]

机构：[1]East China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Engn Res Ctr Biomat, Minist Educ, Shanghai 200237, Peoples R China;[3]Queensland Univ Technol, Australia China Ctr Tissue Engn & Regenerat Med, Brisbane, Qld 4059, Australia;[4]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2018

卷号：6

期号：9

起止页码：2496

外文期刊名：BIOMATERIALS SCIENCE

收录：;EI(收录号：20183505744055);WOS:【SCI-EXPANDED(收录号:WOS:000443267300019)】；

基金：The authors wish to express their gratitude for financial support from the National Natural Science Foundation of China (No. 31330028, No. 31470923), the National Natural Science Foundation of China for Innovative Research Groups (No. 51621002), the International Cooperation Project and Research Project of Shanghai Science and Technique Committee (15520711100, 15DZ1942500), and the Key Project of Logistic Scientific Research of PLA (BWS13C014). This study was also supported by the 111 Project (B14018). The Shanghai Synchrotron Radiation Facility is also gratefully acknowledged.

语种：英文

外文关键词：Bone - Chitosan - Stem cells

摘要：The immunomodulatory property of biomaterials is vital in determining the in vivo fate of implants and tissue regeneration. Bone morphogenetic protein-2 (BMP-2) has been identified as an effective inducer of osteogenic differentiation for bone repair. But it is still unclear how the immunomodulatory effects of materials impact on BMP-2-mediated osteogenesis. Herein, this study aims to investigate the immunoregulatory role of 2-N,6-O-sulfated chitosan (26SCS), a sulfated polysaccharide, in the osteogenetic capacity of BMP-2 and the subsequent effects on bone regeneration. It was noted that 26SCS can robustly activate a moderate pro-inflammatory macrophage response initially, followed by transitioning towards an anti-inflammatory response later on. The immune microenvironment caused by 26SCS could facilitate bone marrow stromal cell (BMSC) chemoattraction. Furthermore, 26SCS significantly up-regulated the expression of BMPR-IA as well as amplified BMP-2-activated BMP/Smad signaling. In addition, the 26SCS-triggered immune microenvironment had a positive effect on proangiogenesis by BMSCs. Our findings suggest that 26SCS may be involved in the induction of a favorable immune microenvironment enhancing crosstalk between immune cells and stem cells undergoing osteogenic differentiation. This relationship is likely responsible for the enhanced bone tissue development observed with 26SCS in an ectopic ossification model.