文献类型：期刊文献

英文题名：An in situ mechanical adjustable double crosslinking hyaluronic acid/ poly-lysine hydrogel matrix: Fabrication, characterization and cell morphology

作者：Guo, Jiahong[1,2,3];Wei, Changzheng[1];Wang, Xiaotong[1];Hou, Yongtai[2];Guo, Weihong[3]

机构：[1]Shanghai Qisheng Biol Preparat Co Ltd, Shanghai 201106, Peoples R China;[2]Shanghai Haohai Biol Technol Co Ltd, Shanghai 200052, Peoples R China;[3]East China Univ Sci & Technol, Sch Mat Sci & Engn, Polymer Proc Lab, Key Lab Preparat & Applicat Ultrafine Mat,Minist, Shanghai 200237, Peoples R China

年份：2021

卷号：180

起止页码：234

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20242616418556);WOS:【SCI-EXPANDED(收录号:WOS:000649643500007)】；

语种：英文

外文关键词：Double-crosslinking hydrogel; Mechanical adjustable; Cell morphology

摘要：Cell fate and morphologies are influenced by the mechanical property of matrix. However, the relevant works about the dynamic adjustable of matrix mechanical property is rare and most of them need extra stimulation, such as the controllable of the degradation. In this study, double crosslinking (DC) hydrogels are fabricated by sequential covalent crosslinking and electrostatic interactions between hyaluronic acid and poly-lysine. Without any extra stimulation or treatment, the compressive stress of DC-hydrogels increases from 22.4 +/- 9.4 kPa to 320.1 +/- 6.6 kPa with the elongation of incubation time in DMEM solution. The change of compressive stress of matrix induced the morphology of L929 fibroblast cells adjusted from the distributed round shape to spheroid cell clusters and finally to spread shape. RNA sequence analysis also demonstrated that the differentially gene expression and GO enrichment between the cells seeded on the DC-hydrogel with different incubation time. In addition, by increasing the electrostatic interactions ratio of the hydrogel, the biodegradation, compressive stress and energy dissipation of the DC-hydrogels were also significantly improved. Therefore, our study provides new and critical insights into the design strategy to achieve DC-hydrogels which can in situ alter cells morphology and open up a new avenue for the application of disease therapy. (c) 2021 Elsevier B.V. All rights reserved.