文献类型：期刊文献

英文题名：Effects of aspirin on expression of iron transport and storage proteins in BV-2 microglial cells

作者：Xu, Yan Xin[1];Du, Fang[3];Jiang, Li Rong[2];Gong, Jing[1,2];Zhou, Yu-Fu[2];Luo, Qian Qian[2];Qian, Zhong Ming[1,2];Ke, Ya[3]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Dept Pharmaceut Sci, Shanghai 200237, Peoples R China;[2]Fudan Univ, Neuropharmacol Lab, Sch Pharm, Shanghai 201203, Peoples R China;[3]Chinese Univ Hong Kong, Fac Med, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China

年份：2015

卷号：91

起止页码：72

外文期刊名：NEUROCHEMISTRY INTERNATIONAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000366780400008)】；

基金：The studies in our laboratories were supported by Hong Kong GRF grant (14106914), HMRF grant (01120146), National 973 Programs (2014CB541604), the General Grant of National Natural Science Foundation of China (NSFC) (31271132-2012, 31371092-2013), and Key Project Grant of NSFC (31330035-2013).

语种：英文

外文关键词：Aspirin (acetylsalicylic acid, ASA); Iron metabolism; Inflammation and lipopolysaccharides (LPS); Transferrin receptor 1 (TfR1); Ferroportin 1 (Fpn1); Tumor necrosis factor-alpha lpha (TNF-alpha); Interleukin-6 (IL-6); Hepcidin

摘要：In the light of recent studies, we hypothesized that aspirin might have the functions to regulate the expression of iron transport proteins and then affect cellular iron levels. To test this hypothesis, we investigated the effects of aspirin on expression of iron uptake protein transferrin receptor 1 (TfR1), iron release protein ferroportin 1 (Fpn1) and iron storage protein ferritin using Western blot analysis and on tumor necrosis factor (TNF)-alpha lpha, interleukin (IL)-6, interleukin (IL)-10 and hepcidin using quantitative real-time PCR in BV-2 microglial cells treated with lipopolysaccharides (LPS). We found that aspirin significantly down-regulated TfR1, while also up-regulated Fpn1 and ferritin expressions in BV-2 microglial cells in vitro. We also showed that TfR1 and Fpn1 expressions were significantly higher, while ferritin contents, IL-6, TNF-alpha and hepcidin mRNA levels were lower in cells treated with aspirin plus LPS than those in cells treated with LPS only. We concluded that aspirin has a negative effect on cell iron contents under 'normal' conditions and could partly reverse LPS-induced-disruption in cell iron balance under in vitro inflammatory conditions. Our findings also suggested that hepcidin might play a dominant role in the control of TfR1 expression by aspirin in the cells treated with LPS. (C) 2015 Elsevier Ltd. All rights reserved.