文献类型：期刊文献

英文题名：Enantioselective esterificaiton of ketoprofen by immobilized lipase in mixed solvent systems

作者：Liu, JM; Xu, JH; Liu, YY; Xu, XS; Yuan, QS

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：1998

卷号：19

期号：12

起止页码：1959

外文期刊名：CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000078261800019)】；

语种：英文

外文关键词：ketoprofen; immobilized lipase; enantioselective esterification; mixed solvent systems

摘要：Enantioselective esterification of racemic ketoprofen [2-(3-benzoylphenyl) propionic acid] was carried out in organic solvents by using celite-adsorbed lipase as the catalyst. Factors influencing the activity of the immobilized lipase had been investigated. Among the seven lipases examined, the esterification activity of Lipase OF(from Candida rugosa) was the highest; the activity of immobilized lipase was improved as compared with free lipase; the optimum volume of buffer(0.1 mol/L Na2HPO4-NaH2PO4, pH 7.0) for enzyme immobilization was 1 mL pergram lipase. Among the acyl receptors such as methanol, ethanol, n-propanol, n-butanol, n-octanol, iso-propanol and 2-ethylene chlorohydrin, n-propanol was the best; In the mixed solvent system composed of a main solvent and a cosolvent, the esterification activity of lipase was higher than that in the single solvent system composed of the corresponding main solvent or cosolvent. The esterification activity of lipase was the highest when using cyclohexane or iso-octane, both of which have high lgP values, as main solvents and toluene as cosolvent.