文献类型：期刊文献

中文题名：缩水甘油丁酸酯的对映选择性酶促水解

英文题名：Enantioselective Enzymatic Hydrolysis of Racemic Glycidyl Butyrate

作者：王阳[1];许建和[1];庄英萍[1];胡英[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室

年份：1999

卷号：25

期号：2

起止页码：209

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

基金：上海市科委基金

语种：中文

中文关键词：缩水甘油丁酸酯;脂肪酶;酶促水解;水解反应

外文关键词：glycidyl butyrate;optical resolution;porcine pancreas lipase;enantioselective hydrolysis

摘要：研究了猪胰脂肪酶催化缩水甘油丁酸酯的对映选择性水解反应，考察了各种因素对酶促水解拆分过程的影响。结果表明，水解产物丁酸的大量生成会抑制脂肪酶的水解活性，至ｐＨ＜５时酶促水解反应几乎停止。采用高浓度的磷酸盐缓冲液可有效地稳定系统的酸碱度，使酶促反应顺利进行。在缓冲液ｐＨ７．５，转速１５０ｒ／ｍｉｎ，反应温度３０℃，每克底物酶用量２５～３５ｍｇ时，外消旋缩水甘油丁酸酯酶促水解转化率达６０％，所得（Ｒ）－酯的对映体过量值（ｅｅ）为８４．７％。
Optical resolution of racemic glycidyl butyrate has been efficiently achieved by lipase catalyzed enantioselective hydrolysis.The reaction conditions have been optimized.The optimum temperature for the enzyme reaction is around 30℃.The optimum initial pH of buffer is 7.5.However,too high concentration of butyric acid produced from the reaction would decrease the pH of the system and inhibit the hydrolytic activity of lipase.This problem was solved by using high concentration of phosphate as buffer system.The optical purity of the finally recovered ( R ) glycidyl butyrate was about 85% ee.