文献类型：期刊文献

英文题名：Bioreduction of methyl o-chlorobenzoylformate at 500 g L^-1 without external cofactors for efficient production of enantiopure clopidogrel intermediate

作者：Ni, Yan[1];Pan, Jiang[1];Ma, Hong-Min[1];Li, Chun-Xiu[1];Zhang, Jie[1];Zheng, Gao-Wei[1];Xu, Jian-He[1]

机构：[1]E China Univ Sci & Technol, Lab Biocatalysis & Synthet Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2012

卷号：53

期号：35

起止页码：4715

外文期刊名：TETRAHEDRON LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000307601300023)，CCR-EXPANDED(收录号:WOS:000307601300023)】；

基金：This work was financially supported by the National Natural Science Foundation of China (No. 20902023 & 31071604), Ministry of Science and Technology, P.R. China (No. 2009CB724706, 2011CB710800 & 2011AA02A210), Innovation Program of Shanghai Municipal Education Commission (No. 11CXY24) and China National Special Fund for State Key Laboratory of Bioreactor Engineering (No. 2060204).

语种：英文

外文关键词：Asymmetric reduction; Aldo-keto reductase; Whole-cell catalyst; Methyl (R)-o-chloromandelate; Clopidogrel

摘要：Biocatalytic reduction of methyl o-chlorobenzoylformate (CBFM) provides a green and direct access to methyl (R)-o-chloromandelate [(R)-CMM], an intermediate for a platelet aggregation inhibitor named clopidogrel. As much as 500 g L-1 of CBFM was stoichiometrically converted into enantiopure (R)-CMM at 20 degrees C by using a whole-cell catalyst coexpressing an aldo-keto reductase from Bacillus sp. and a glucose dehydrogenase (GDH). In addition to the high productivity of 812 g L-1 d(-1), this new whole-cell reduction is attractive by eliminating the need of an added external cofactor. (C) 2012 Elsevier Ltd. All rights reserved.