文献类型：期刊文献

英文题名：Sequential Heterotrophy-Dilution-Photoinduction Cultivation of Haematococcus pluvialis for efficient production of astaxanthin

作者：Wan, Minxi[1];Zhang, Zhen[1];Wang, Jun[2];Huang, Jianke[1];Fan, Jianhua[1];Yu, Anquan[2];Wang, Weiliang[1];Li, Yuanguang[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Jiaxing Zeyuan Bioprod Ltd Co, Jiaxing 314006, Peoples R China

年份：2015

卷号：198

起止页码：557

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20154101352445);WOS:【SCI-EXPANDED(收录号:WOS:000363487500071)】；

基金：This research was funded by National High Technology Research and Development Program of China (2015AA020602), National Natural Science Foundation of China (31500062), National Key Technologies R&D Program (2011BAD23B04), National Basic Research Program of China (973 Program: 2011CB200903 & 2011CB200904), China Postdoctoral Science Foundation (2013M530183 & 2014T70400), the Fundamental Research Funds for the Central Universities (222201414024). The authors declared that they have no conflicts of interest to this work.

语种：英文

外文关键词：Haematococcus pluvialis; Heterotrophy; Dilution; Photoinduction; Astaxanthin

摘要：A novel cultivation strategy called "Sequential Heterotrophy-Dilution-Photoinduction" was successfully applied in the cultivation of Haematococcus pluvialis to produce astaxanthin effectively. Cells were first cultivated heterotrophically to achieve a high cell density, then were diluted to a suitable concentration and switched to a favorable environment for cells acclimation. Finally, the culture was transferred to high light environment for astaxanthin accumulation. By this strategy, the dry cell weight of 26 g/L and biomass productivity of 64.1 mg/L/h were obtained in heterotrophy stage which surpassed ever before reported in literatures. Meanwhile, the cells could accumulate considerable astaxanthin up to 4.6% of dry cell weight after 10 days of photoinduction. Furthermore, the application prospects of the strategy were confirmed further by outdoor experiments. Therefore, this novel strategy provided a promising approach for high-efficient production of natural astaxanthin from H. pluvialis to meet the huge demand of this high value product. (C) 2015 Elsevier Ltd. All rights reserved.