文献类型：期刊文献

英文题名：Quantitative determination of four diterpenoids in radix salviae miltiorrhizae using LC-MS-MS

作者：Hu, P; Luo, GA; Zhao, ZZ; Jiang, ZH

机构：[1]Hong Kong Baptist Univ, Sch Chinese Med, Kowloon, Hong Kong, Peoples R China;[2]E China Univ Sci & Technol, Modern Engn Ctr Trad Chinese Med, Sch Chem & Pharmaceut, Shanghai 200237, Peoples R China;[3]Tsinghua Univ, Anal Ctr, Beijing 100084, Peoples R China

年份：2005

卷号：53

期号：6

起止页码：705

外文期刊名：CHEMICAL & PHARMACEUTICAL BULLETIN

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000229861400025)】；

语种：英文

外文关键词：HPLC-ESI-MS-MS; quantitative analysis; Salvia miltiorrhiza; diterpenoid; tanshinone

摘要：An HPLC-ESI-MS-MS method has been developed for the quantitative determination of four diterpenoids, dihydrotanshinone I, cryptotanshinone, tanshinone I, and tanshinone IIA in Radix Salviae Miltiorrhizae (RSM, the root of Salvia miltiorrhiza B-GE.). The diterpenoids were chromatographically separated on a C18 HPLC column, and the quantification of these diterpenoids was based on the fragments of [M+H](+) under collision-activated conditions and in Selected Reaction Monitioring (SRM) mode. The quantitative method was validated, and the mean recovery rates from fortified samples (n=5) of dihydrotanshinone I, cryptotanshinone, tanshinone I, and tanshinone IIA were 95.0%, 97.2%, 93.1 %, and 95.9% with variation coefficient of 6.0%, 4.3%, 3.7%, and 4.2%, respectively. The established method was successfully applied to the quality assessment of seven batches of RSM samples collected from different regions of China.