文献类型：期刊文献

英文题名：Characterization of hepatotoxic effects induced by pyraclostrobin in human HepG2 cells and zebrafish larvae

作者：Wu, Mengqi[1]; Bian, Jinhao[1]; Han, Shuang[1]; Zhang, Cheng[2]; Xu, Wenping[1]; Tao, Liming[1]; Li, Zhong[1,3]; Zhang, Yang[1]

机构：[1] Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China; [2] Department of Pathology, UT Southwestern Medical Center, Dallas, TX, 75390, United States; [3] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China

年份：2023

卷号：340

外文期刊名：Chemosphere

收录：EI(收录号：20233414583207)

语种：英文

外文关键词：Activation analysis - Disease control - Mitochondria

摘要：Pyraclostrobin is a highly effective and broad-spectrum strobilurin fungicide. With the widespread use of pyraclostrobin to prevent and control crop diseases, its environmental pressure and potential safety risks to humans have attracted much attention. Herein, the toxicological risks of pyraclostrobin toward HepG2 cells and the mechanisms of intoxication in vitro were investigated. The liver toxicity of pyraclostrobin in zebrafish larvae was also evaluated. It was found that pyraclostrobin induced DNA damage and reactive oxygen species generation in HepG2 cells, indicating the potential genotoxicity of pyraclostrobin. The results of fluorescent staining experiments and the expression of cytochrome c, Bcl-2 and Bax demonstrated that pyraclostrobin induced mitochondrial dysfunction, resulting in cell apoptosis. Monodansylcadaverine staining and autophagy marker-related proteins LC3, p62, Beclin-1 protein expression showed that pyraclostrobin promoted cell autophagy. Furthermore, immunoblotting analysis suggested that pyraclostrobin induced autophagy accompanied with activation of adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK)/mTOR signaling pathway. Visualization of zebrafish liver and oil red staining indicated that pyraclostrobin could induce liver degeneration and liver steatosis in zebrafish. Collectively, these results help to better understand the hepatotoxicity of pyraclostrobin and provide a scientific basis for its safe applications and risk control. ? 2023 Elsevier Ltd