文献类型：期刊文献

英文题名：Improvement of chaetominine production by tryptophan feeding and medium optimization in submerged fermentation of Aspergillus fumigatus CY018

作者：Zhang, Yu-peng[1,2]; Jiao, Rui-hua[3]; Lu, Yan-hua[1,2]; Yao, Ling-yun[1,4]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Box 283#, 130 Meilong Road, Shanghai, 200237, China; [2] Shanghai Collaborative Innovation Center for Biomanufacturing Technology, 130 Meilong Road, Shanghai, 200237, China; [3] State Key Laboratory of Pharmaceutical Biotechnology, Institute of Functional Biomolecules, Nanjing University, Nanjing, 210093, China; [4] School of Perfume and Aroma Technology, Shanghai Institute of Technology, 100 Hai Quan Road, Shanghai, 201418, China

年份：2016

卷号：3

期号：1

外文期刊名：Bioresources and Bioprocessing

收录：EI(收录号：20224513087545)

语种：英文

外文关键词：Amino acids - Biotechnology - Chlorine compounds - Fermentation - Iron compounds - Magnesium compounds

摘要：Background: Chaetominine (CHA) is a novel alkaloid with excellent medicinal activities produced by Aspergillus fumigatus CY018. However, its further application has been severely restricted by the low production yield. In this work, the fermentation titer of CHA was investigated by medium composition optimization and amino-acid addition strategies. Results: Under the optimized conditions of sucrose 115.03g/L, ammonium acetate 3.98g/L, d-tryptophan 3.84g/L, KH2PO4 1.5g/L, FeSO4·7H2O 0.02g/L, MgSO4·7H2O 0.7g/L, sodium glutamate 3g/L, sodium tartrate 1.5g/L, and CaCl2 0.045g/L), a CHA production yield of 55.92mg/L was obtained, which increased significantly (3.99-fold) as compared with the unoptimized basal medium. Scale-up fermentation was carried out in a 5-L bioreactor based on the shake-flask fermentation results, maximum CHA yield of 48.53mg/L was obtained at an air flow rate of 2.0±0.1 VVM and an agitation rate of 400rpm. Conclusion: These results demonstrated that medium composition optimization and amino-acid addition were useful strategies for improving CHA production via biotechnological process. The methods in this work would be useful for the biotechnological production of CHA from A. fumigatus. ? 2016, The Author(s).