文献类型：期刊文献

英文题名：Peptide-laden mesoporous silica nanoparticles with promoted bioactivity and osteo-differentiation ability for bone tissue engineering

作者：Luo, Zuyuan[1,2];Deng, Yi[1,2];Zhang, Ranran[3];Wang, Mengke[1];Bai, Yanjie[4,5];Zhao, Qiang[4,5];Lyu, Yalin[3];Wei, Jie[6];Wei, Shicheng[1,2]

机构：[1]Peking Univ, Dept Oral & Maxillofacial Surg, Sch & Hosp Stomatol, Beijing 100081, Peoples R China;[2]Peking Univ, Acad Adv Interdisciplinary Studies, Ctr Biomed Mat & Tissue Engn, Beijing 100871, Peoples R China;[3]Capital Med Univ, Beijing Anzhen Hosp, Dept Stomatol, Beijing 100029, Peoples R China;[4]China Med Univ, Aviat Gen Hosp, Dept Stomatol, Beijing 100012, Peoples R China;[5]Chinese Acad Sci, Being Inst Translat Med, Beijing 100012, Peoples R China;[6]E China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China

年份：2015

卷号：131

起止页码：73

外文期刊名：COLLOIDS AND SURFACES B-BIOINTERFACES

收录：;EI(收录号：20151900836156);WOS:【SCI-EXPANDED(收录号:WOS:000357354800010)】；

基金：This work was supported by Beijing Natural Science Foundation (No. 7132124) and Peking University's 985 Grant.

语种：英文

外文关键词：Mesoporous silica nanoparticles; Peptide; Bioactivity; Osteo-differentiation; Bone tissue engineering

摘要：Combination of mesoporous silica materials and bioactive factors is a promising niche-mimetic solution as a hybrid bone substitution for bone tissue engineering. In this work, we have synthesized biocompatible silica-based nanoparticles with abundant mesoporous structure, and incorporated bone-forming peptide (BFP) derived from bone morphogenetic protein-7 (BMP-7) into the mesoporous silica nanoparticles (MSNs) to obtain a slow-release system for osteogenic factor delivery. The chemical characterization demonstrates that the small osteogenic peptide is encapsulated in the mesoporous successfully, and the nitrogen adsorption-desorption isotherms suggest that the peptide encapsulation has no influence on mesoporous structure of MSNs. In the cell experiment, the peptide-laden MSNs (p-MSNs) show higher MG-63 cell proliferation, spreading and alkaline phosphatase (ALP) activity than the bare MSNs, indicating good in vitro cytocompatibility. Simultaneously, the osteogenesis-related proteins expression and calcium mineral deposition disclose enhanced osteo-differentiation of human mesenchymal stem cells (hMSCs) under the stimulation of the p-MSNs, confirming that BFP released from MSNs could significantly promote the osteogenic differentiation of hMSCs, especially at 500 mu g/mL of p-MSNs concentration. The peptide-modified MSNs with better bioactivity and osteogenic differentiation make it a potential candidate as bioactive material for bone repairing, bone regeneration, and bio-implant coating applications. (C) 2015 Elsevier B.V. All rights reserved.