文献类型：期刊文献

英文题名：Synthesis of a molecularly imprinted polymer using MOF-74(Ni) as matrix for selective recognition of lysozyme

作者：Li, Jie[1];Ma, Miaoxia[1];Zhang, Chenhui[1];Lu, Rui[1];Zhang, Lingyi[1];Zhang, Weibing[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2020

卷号：412

期号：26

起止页码：7227

外文期刊名：ANALYTICAL AND BIOANALYTICAL CHEMISTRY

收录：;EI(收录号：20203409065333);WOS:【SCI-EXPANDED(收录号:WOS:000560639800002)】；

基金：We gratefully acknowledged the support of the Science and Technology Commission of Shanghai Municipality (19142201100), the National Natural Science Foundation of China (21974045), and the Fundamental Research Funds for the Central Universities (50321102017022).

语种：英文

外文关键词：Molecularly imprinted polymer; Metal organic framework; Surface imprinting; Lysozyme; Polydopamine

摘要：A molecularly imprinted polymer and metal organic framework were combined to prepare protein imprinted material. MOF-74(Ni) was used as a matrix to prepare surface-imprinted material with lysozyme as a template and polydopamine as an imprinting polymer. MOF-74(Ni) not only provides a large surface area (150.0 m(2)/g) to modify the polymer layer with more recognition sites (Wt(Ni) = 42.24%), but also facilitates the immobilization of lysozyme by using the chelation between Ni(2+)of the MOF-74(Ni) and protein. The thin polydopamine layer (10 nm) of the molecularly imprinted material (named MOF@PDA-MIP) enables surface imprinting. Benefiting from the thin polymer layer, MOF@PDA-MIP reached adsorption equilibrium within 10 min. The maximum adsorption capacity reaches 313.5 mg/g with the highest imprinting factor (IF) of 7.8. The specific recognition sites can distinguish target lysozyme from other proteins such as egg albumin (OVA), bovine serum albumin (BSA) and ribonuclease A (RNase A). The material was successfully applied to separation of lysozyme from egg white.