文献类型：期刊文献

英文题名：Ultrasound-Responsive Hydrogel Incorporated with TGF-β Mimetic Peptides for Endometrium Recovery to Restore Fertility

作者：Qin, Jinlong[1,2];Sun, Min[2];Cheng, Jiajing[2];Jiang, Huici[2];Lv, Mingchen[1];Jing, Jianxing[1];Chen, Ran[1];Fan, Zhen[1,2];Du, Jianzhong[1,2,3]

机构：[1]Tongji Univ, Sch Mat Sci & Engn, Dept Polymer Mat, Shanghai 201804, Peoples R China;[2]Tongji Univ, Clin Res Ctr Anesthesiol & Perioperat Med, Dept Gynaecol & Obstet, Shanghai Key Lab Anesthesiol & Brain Funct Modulat, Shanghai 200434, Peoples R China;[3]East China Univ Sci & Technol, Sch Mat Sci & Engn, Shanghai 200237, Peoples R China

年份：2024

卷号：16

期号：43

起止页码：57963

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20244317232587);WOS:【SCI-EXPANDED(收录号:WOS:001338852700001)】；

基金：This work was financially support by the National Natural Science Foundation of China (Grants 52222306, 22075212, 22335005, 22305177, and 21925505), Shanghai International Scientific Collaboration Fund (23520710900), Shanghai Hongkou District Science and Technology Commission (2022-31), Shanghai Hongkou District Public Health Key Support Discipline Construction Project (HKGWFC 202401), Postdoctoral Fellowship Program of China Postdoctoral Science Foundation (GZB20230517 and 2022M720107), Shanghai "Super Postdoc" Incentive Plan (2022568), Shanghai Rising-Star Program (Sailing, 23YF1433000), and the Fundamental Research Funds for the Central Universities. J.D. is the recipient of a 5-year National Science Fund for Distinguished Young Scholars.

语种：英文

外文关键词：pregnancy; endometrial injury; hydrogel; peptide; wound healing

摘要：Unavoidable damage to the basal layer of the endometrium has a huge negative impact on a woman's reproductive health and menstrual cycle. However, it is difficult for medicine to penetrate a series of biological barriers toward the basal layer in the deeper area of the endometrium. To meet this challenge, we developed an ultrasound-responsive hydrogel incorporated with a transforming growth factor-beta (TGF-beta) mimetic peptide to enhance pregnancy outcomes by restoring the function of a wounded endometrium due to its deep-tissue-penetration capability. In vitro studies revealed that the TGF-beta-mimetic-peptide-loaded hydrogel could achieve 64.35% of cell migration under ultrasound stimulation even in phosphate-buffered saline of pH 6.0. Upon in situ sonication at the uterus, carboxymethyl chitosan can be released from degraded hydrogel to open tight junctions with reduced interstitial pressure by ultrasound to promote deep penetration. Rat studies revealed that the penetration capability of TGF-beta-mimetic-peptide-loaded hydrogel with sonication was 1.6 times higher than that of the control group. Besides the rat uterine model, ex vivo human uterine tissue was also collected and imaged, demonstrating up to similar to 700 mu m of tissue penetration depth. In addition, compared to control groups, effective uterus recovery without intrauterine stenosis and endometrial cavity fluid was observed from rats with severe uterine injury treated by TGF-beta-mimetic-peptide-loaded hydrogel. In addition, fertility restoration in the endometrial injury model was observed after treatment with such an ultrasound-responsive hydrogel incorporated with TGF-beta mimetic peptides. Overall, this work provides an effective approach to treating endometrial injury for enhanced pregnancy outcomes.