文献类型：期刊文献

英文题名：Core-shell magnetic microporous covalent organic framework with functionalized Ti(iv) for selective enrichment of phosphopeptides

作者：Ding, Fengjuan[1];Zhao, Yameng[1];Liu, Haiyan[1];Zhang, Weibing[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China

年份：2020

卷号：145

期号：12

起止页码：4341

外文期刊名：ANALYST

收录：;EI(收录号：20204209366280);WOS:【SCI-EXPANDED(收录号:WOS:000542708600029)】；

基金：This research was financially supported by the National Natural Science Foundation of China (No. 21974045), Science and Technology Commission of Shanghai Municipality (No. 19142201100) and the Fundamental Research Funds for the Central Universities (No. 50321102017022).

语种：英文

外文关键词：Magnetism - Casein - Silicon - Binding sites - Silica - Peptides - Shells (structures) - Titanium compounds

摘要：Selective, sensitive and efficient phosphopeptide enrichment is significant in understanding phosphorylation-regulated processes and finding potential biomarkers. Magnetic materials have significant advantages for the separation and enrichment of modified peptides and proteins. In this work, we fabricated a core-shell magnetic Ti4+-functionalized covalent organic framework composite (denoted as Fe3O4@SiO2@TpPa-Ti4+) to selectively capture phosphopeptides in biosamples. The specific porous structure makes high surface area, which provides space and more affinity sites to binding titanium ions. The loading capacity of titanium ions was as high as 14% (wt%). Calculated by the MS results of alpha-casein tryptic digests, the binding capability could reach 200 mg g(-1). Fe3O4@SiO2@TpPa-Ti(4+)nanoparticles also demonstrate high sensitivity (above 0.2 fmol mu L-1) and selectivity (alpha-casein : BSA = 1 : 5000). 1083 phosphopeptides were identified within two LC-MS/MS replicates with 91.8% specificity (phosphopeptides/all identified peptides). These MS results demonstrate that with the aid of the magnet, this method based on the magnetic titanium-functionalized covalent organic framework composite is applicable to achieve rapid, selective and efficient phosphopeptide analysis.