文献类型：期刊文献

英文题名：Occurrence of human-pathogenic Enterocytozoon bieneusi, Giardia duodenalis and Cryptosporidium genotypes in laboratory macaques in Guangxi, China

作者：Ye, Jianbin[1];Xiao, Lihua[2];Li, Jian[3];Huang, Weiyi[3];Amer, Said E.[4];Guo, Yaqiong[1,2];Roellig, Dawn[2];Feng, Yaoyu[1]

机构：[1]E China Univ Sci & Technol, Sch Resources & Environm Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Ctr Dis Control & Prevent, Atlanta, GA 30329 USA;[3]Guangxi Univ, Coll Anim Sci & Technol, Nanning 530005, Guangxi, Peoples R China;[4]Kafr El Sheikh Univ, Fac Sci, Dept Zool, Kafr Al Sheikh 33516, Egypt

年份：2014

卷号：63

期号：1

起止页码：132

外文期刊名：PARASITOLOGY INTERNATIONAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000328915400019)】；

基金：This work was supported by the National Natural Science Foundation of China (31229005 and 31110103901), National Special Fund for State Key Laboratory of Bioreactor Engineering, China (No. 2060204), Fundamental Research Funds for the Central Universities, China, and Centers for Disease Control and Prevention.

语种：英文

外文关键词：Cryptosporidium; Giardia; Enterocytozoon bieneusi; Cyclospora; Macaca fascicularis

摘要：Captive nonhuman primates have been identified as common hosts of Enterocytozoon bieneusi, Giardia duodenalis, Cryptosporidium hominis, and Cyclospora spp., thus are potential reservoirs of some enteric parasites in humans. However, few studies have examined the source and human-infective potential of enteric parasites in laboratory nonhuman primates. In the present work, 205 fecal specimens were collected from three groups of captive Macaca fascicularis kept in different densities in a laboratory animal facility in Guangxi, China, and examined by PCR for E. bieneusi, G. duodenalis, Cryptosporidium spp., and Cyclospora spp. The infection rates of E. bieneusi and G. duodenalis were 11.3% and 1.2% in Group 1 (young animals kept individually; n = 168), 72.2% and 11.1% in Group 2 (young animals kept in groups; n = 18), and 31.6% and 53% in Group 3 (adults kept in groups; n = 19), respectively. Sequence analysis of PCR products showed the presence of five E. bieneusi genotypes, with genotype D (in 16/36 genotyped specimens) and a new genotype (in 15/36 genotyped specimens) as the dominant genotypes. All five E. bieneusi genotypes belonged to the zoonotic group (Group 1). The G. duodenalis genotypes (assemblages All and B) in five specimens and C. hominis subtype (IdA14) in one specimen were also known human-pathogens, although the Cyclospora seen in one animal appeared to be unique to macaque monkeys. The higher infection rate in younger animals reared in groups and common occurrence of zoonotic genotypes indicated that human-pathogenic E. bieneusi could be transmitted efficiently in captive nonhuman primates, and group-housing was a risk factor for transmission of zoonotic pathogens in young nonhuman primates in research facilities. Published by Elsevier Ireland Ltd.