文献类型：期刊文献

英文题名：Effect of dietary benzoxadiazole on larval development, cuticle enzyme and antioxidant defense system in housefly (Musca domestica L.)

作者：Huang, Qingchun[1,2];Liu, Manhui[1];Feng, Jun[1];Liu, Yang[1]

机构：[1]E China Univ Chem Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, State Key Lab Bioactivat Engn, Shanghai 200237, Peoples R China

年份：2008

卷号：90

期号：2

起止页码：119

外文期刊名：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000253359400007)】；

语种：英文

外文关键词：dietary benzoxadiazole; development regulation; cuticle enzyme; antioxidant defense system; Musca domestica (L.)

摘要：The toxicity and influence on chronic development regulation of dietary benzoxadiazole as well as the subsequent action on cuticle enzyme and antioxidant defense system in feed-thru housefly larvae are investigated. Dietary benzoxadiazole shows limited larvicidal activity and weak interference on larval pupation, but strong blockage against the succedent eclosion process. It does not change the content ratio of protein/chitin in the larval cuticle, but strongly regulates the constituents of cuticle proteins. Moreover, chitinase activities in the integument of third-instar larvae, in vivo, are enhanced and gradually decreased whereas phenoloxidase activities are inhibited and the inhibitory rates are gradually increased. Glutathione S-transferase activities are strongly improved whilst peroxidase activities decrease from about 42.25% to 17.36%, catalase activities decrease from about 80.31% to 27.98% and superoxide dismutase activities are almost unchanged during the different treatment procedures. Peroxidase SDS-PAGE analysis shows that band photodensities of 200.0 and 10.3 kDa proteins in feed-thru larvae are significantly weaker than the corresponding control band. Results suggest that dietary benzoxadiazole might exert strong regulation on larvae cuticle metabolism, interfere with cuticle enzymatic browning and protein sclerotization and weaken the self-protection of larvae against endogenetic oxidative damages. (c) 2007 Elsevier Inc. All rights reserved.