文献类型：期刊文献

英文题名：Metabolomics and network pharmacology reveal the anti-inflammatory core substances and mechanisms of a new cultivar of high-polyphenol lettuce

作者：Lu, Qianshan[1];Tian, Wenxuan[1];Cheng, Yanzhe[1];Chen, Zitian[1];Cao, Yu[1];Zhao, Haiying[2];Tong, Zhou[2];Chang, Yaning[1];Zhou, Yingjun[1]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]Zhejiang Jiameishan Plant Technol Co, Shanghai, Peoples R China

年份：2025

卷号：105

期号：10

起止页码：5419

外文期刊名：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001478094300001)】；

基金：The material in the graphical abstract was supported by Figdraw (www.figdraw.com).

语种：英文

外文关键词：lettuce; polyphenols; metabolomics; anti-inflammation; network pharmacology

摘要：BACKGROUNDDiet plays a significant role in mitigating inflammation, with lettuce and its polyphenols showing potential for improving inflammatory conditions. However, research on the material basis and mechanisms of action remains unclear.RESULTSThis study investigated the primary components and anti-inflammatory effects of the new lettuce cultivar Binfen-1, which is rich in polyphenols and may offer enhanced health benefits. There were 65 metabolites with significant changes in Binfen-1 by metabolomics, comprising mainly polyphenols. Meanwhile, the high-polyphenol lettuce extract (HPLE) from Binfen-1 demonstrated superior antioxidant and anti-inflammatory effects in vitro. Moreover, HPLE showed a significant improvement in mice with dextran sodium sulfate-induced ulcerative colitis. By integrating network pharmacology with high-performance liquid chromatographic quantitative results, isoquercitrin and chlorogenic acid were identified as the core active substances in HPLE, and the PI3K-Akt, MAPK, and JAK-STAT pathways were the key anti-inflammatory pathways for HPLE, supported by mRNA validation and molecular docking predictions.CONCLUSIONThe core anti-inflammatory substances in HPLE were isoquercitrin and chlorogenic acid, with the key anti-inflammatory pathways being the PI3K-Akt, MAPK, and JAK-STAT pathways. These findings substantially propel the understanding of the therapeutic potential of lettuce in the treatment of inflammatory diseases. (c) 2025 Society of Chemical Industry.