文献类型：期刊文献

英文题名：Monitoring Autophagy with Atg4B Protease-Activated Aggregation-Induced Emission Probe

作者：Lyu, Yanting[1,2];Chen, Xiaoyan[3];Wang, Qi[1,2];Li, Qiang[1,2];Wang, Quan[4];Li, Xiangyu[1,2];Zhu, Zhirong[1,2];Yan, Chenxu[1,2];Zhao, Xiaolei[1,2];Zhu, Wei-Hong[1,2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Frontiers Sci Ctr Materiobiol & Dynam Chem,Fering, Key Lab Adv Mat,Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Frontiers Sci Ctr Materiobiol & Dynam Chem,Fering, Inst Fine Chem,Joint Int Res Lab Precis Chem & Mo, Shanghai 200237, Peoples R China;[3]Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Pathol, Shanghai 200025, Peoples R China;[4]Shanghai Jiao Tong Univ, Sch Biomed Engn, Renji Hosp, State Key Lab Oncogenes & Related Genes, Shanghai 200030, Peoples R China

年份：2022

卷号：32

期号：6

外文期刊名：ADVANCED FUNCTIONAL MATERIALS

收录：;EI(收录号：20214411101392);WOS:【SCI-EXPANDED(收录号:WOS:000711452400001)】；

基金：Y.L. and X.C. contributed equally to this work. This work was supported by National Key Research and Development Program (2021YFA090164), NSFC Science Center Program (21788102), NSFC/China (91959202, 21974047, and 21622602), Pujiang Talents Plan (19PJ1402300), China Postdoctoral Science Foundation (2020M671328), Postdoctoral Science Foundation of Jiangsu Province (2020Z189), Shanghai Municipal Science and Technology Major Project (2018SHZDZX03).

语种：英文

外文关键词：aggregation-induced emission; Atg4B; autophagy; real-time sensing

摘要：Autophagy is crucial in the physical development and pathogenesis of disease. Monitoring autophagy is still limited by no real-time and poor specificity. Herein, the authors report studies of Atg4B enzyme as a biomarker for autophagy and the development of an Atg4B-responsive aggregation-induced emission (AIE) probe, termed QM-GFTN. Particularly, the incorporated peptide unit of GFTN endows good dispersion, but the state can be reversed when the hydrophilic peptide is cleaved by the Atg4B, thus realizing a light-up fluorescence with high signal/noise (S/N) ratio. The probe exhibits excellent selectivity to Atg4B, and can respond to its activity in solution and living cells, thus efficiently distinguishing autophagy-active from autophagy-inactive states, greatly shortening the detection time comparing to the traditional methods. The fluorescence imaging of cells and autophagy-inhibitor studies indicate that QM-GFTN is specifically cleaved by Atg4B, and rules out the possibility of its self-aggregation. By grafting Atg4B-cleavable peptide to AIE fluorophore, the peptide-based probe can carry out real-time visualization of Atg4B activity with guaranteeing high specificity, serving as a great breakthrough to the detection of autophagy process in various autophagic cells, animal tissues, and even human pathological tissues.